Investigation and Analysis on Infection of Chicken Coccidia in Some Regions of Zhejiang Province
In order to understand the infection status of chicken coccidia in some regions of Zhe-jiang province,and to identify the new species,Eimeria latan,Eimeria nagambie and Eimeria zaria,349 fresh chicken feces were collected from 7 farms in Hangzhou,Lishui and Jiaxing.The number of coccidia oocysts per gram of feces was examined and counted by McMaster counting method.The species of coccidia in positive samples were determined by microscopic observation and identified by PCR.Subsequently,the infection of chicken coccidia was analyzed according to regional sources,breeding methods,uses and age.The result showed that the total infection rate of chicken coccidia in the investigated area was 72.78％(254/349),and seven species of Eimeria and Eimeria nagambie were detected,among which the dominant species was Eimeria acervuli-na,mixed infection were found in all farms.The PCR and sequence analysis results showed that four of the seven farms were infected with suspected Eimeria nagambie,the Eimeria nagambie of farm A had the closest homology with the COI gene of the Australian strain LR877717.1,the homology is 99.99％,and clustered into a branch.The results showed that the overall infection rate of chicken coccidia in Zhejiang province was high,and the field infection of E.acervulina was particularly serious.Some chicken farms in some areas of Zhejiang Province have been infec-ted with suspected new species of coccidiosis Eimeria nagambie
万方数据
维普
