The aim is to study the pathogenic mechanism of porcine epidemic diarrhea virus(PEDV)by transcriptome analysis of the jejunum of two-day-old piglets infected with PEDV.Three two-day-old piglets were infected with the PEDV HB strain(PEDV-HB),which belongs to the currently prevalent G2b subtype.At 18 h post-infection,jejunum tissues were collected from piglets for pathology,PEDV detection,RNA-seq and transcriptome analysis.Finally;the transcriptome results were verified by quantitative real-time PCR(RT-qPCR).The result showed that the jejunal villi of piglets infected PEDV-HB were significantly shed and shortened;and was mainly distributed on the surface of intestinal villi.GO and KEGG enrichment analysis showed that differentially expressed genes(DEGs)were mainly related to immune response and inflammatory response,and were enriched in several classical pathways,such as cytokine-cyto-kine receptor interaction,Toll-like receptor signaling pathway,fat and glucose absorption and metabolism.PEDV infection of the jejunum of piglets activates the host immune response and as-sociated antiviral signaling pathways and induces a cytokine storm as well as dysfunctions in fat and glucose absorption and metabolism.In all,this research can lay the foundation for the study of pathogenesis mechanism of PED.
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