摘要
鸡滑液囊支原体(Mycoplasma synoviae,MS)是现在规模化禽养殖业中危害最大的支原体之一,同时也出现了严重的耐药性.为了解鸡滑液囊支原体对各种药物的耐药现状.本课题参考CLSI和EUCAST中制定流行病学/野生型临界值(epidemiological/wildtype cut-off values,ECOFFs/COWT)的方法,通过查找国内外相关文献报道,收集汇总近5年不同地区、不同来源的MS对各种药物的最小抑菌浓度(minimum inhibitory concentration,MIC)数据,应用肉眼观察法、ECOFFinder软件分析法和正态化耐药解释法(NRI)综合分析从而得到ECOFFs,随后计算相应的耐药率.结果显示,MS对泰乐菌素、替米考星、泰万菌素、多西环素、土霉素、金霉素、泰妙菌素、林可霉素、氟苯尼考和大观霉素的ECOFF值分别为1、0.5、0.5、2、4、2、2、2、8和4μg·mL-1.在CLSI和EUCAST缺乏MS对各种抗菌药的敏感性折点下,本课题为临床合理用药,耐药性工作的开展,减少MS耐药性的产生以及治疗与防控提供了理论基础与科学依据.
Abstract
Mycoplasma synoviae(MS)is one of the most detrimental mycoplasmas in the large-scale poultry industry.Meanwhile,the development of antimicrobial resistance to MS is serious currently.In order to understand the current situation of antimicrobial resistance to MS,the re-search was conducted in reference to the method of establishing epidemiological/wildtype cut-off values(ECOFFs/COWT)in CLSI and EUCAST by collecting and summarizing MIC from differ-ent regions and sources in recent 5 years after searching relevant literature reports at home and abroad,and then the ECOFFs of various antimicrobial agents to MS were obtained through com-prehensive analysis using visual estimation,ECOFFFinder software analysis and Normalized Drug Resistance Interpretation(NRI).Subsequently,the corresponding drug resistance rates were calculated.It showed that the ECOFFs of MS to tylosin,tilmicosin,tylvalosin,doxycyc-line,oxytetracycline,chlortetracycline,tiamulin,lincomycin,florfenicol and spectinomycin were 1,0.5,0.5,2,4,2,2,2,8 and 4 μg·mL-1 respectively.In absence of sensitive breakpoint of MS in CLSI and ECUAST,the theoretical basis and scientific evidence are provided by this re-search for the rational use of drugs in clinical practice,development of antimicrobial resistance work for monitoring and reducing it,and also beneficial for the treatment and control of MS.
基金项目
国家重点研发计划(2021YFD1800600)
国家自然科学基金(32172914)
中央高校自主创新基金(2662022DKYJC005)
湖北省自然科学基金(2021CFA016)
现代农业产业技术体系项目(CARS-41)
维普
万方数据