Viola yedoensis Makino Improves the Growth Performance,Meat Quality,and Gut Microbiota of Broilers Exposed to Heat Stress
This study assessed the mitigation effects of Viola yedoensis Makino(VYM)on the growth performance,meat quality,and gut microbiota of AA broilers under heat stress.Sixty 1-day-old male AA broilers were randomly allotted into 6 treatments.The NT group was fed with a standard diet at normal temperature.The NT+VYM-H group was fed the diet supplemented with 4.5％VYM at normal temperature;The HS group was fed with a standard diet under heat stress;The HS+VYM-L,HS+VYM-M,and HS+VYM-H groups were fed diets containing 0.5％,1.5％,and 4.5％VYM under heat stress.All broilers were slaughtered at 42 days old.Growth performance,serrum biochemistry,meat quality,duodenum morphorlogy,related genes and composition of gut microbe were analyzed.Broilers fed with 1.5％VYM(w/w)had higher average daily weight gain and a lower feed intake/body weight gain(F/G)ratio under heat stress(P＜0.05).Under heat stress,VYM supplementation reduced serum HSP70 levels(P＜0.05),while the supplementation of 0.5％VYM increased duodenal lipase activity(P＜0.05),and up-regulated myogenic regulatory-related mRNA(Pax3,Pax7,Myog,Myod,Myf5)expression in the breast muscle of broilers,and upregulated digestive-related mRNA(Ppara,Fatp1,B0at1,Pept1,Cat1,Eaat3)expression in the duodenum(P＜0.05).Under heat stress,1.5％VYM supplementation decreased shearing stress and the color of meat(P＜0.05).In the cecum,VYM remarkably improved the richness of intestinal microflora(P＜0.05)and increased the a-bundance of Bacteroides,which was significantly positively related to meat pH(45 min).It also lowered the abundance of Subdoligranulum,which was significantly negatively correlated with duodenal amylase activity.In conclusion,under the conditions of this study,the degradation of growth performance and meat quality caused by heat stress can be reduced and the balance of in-testinal flora of broilers can be regulated by the supplementation of 1.5％and 4.5％Viola ye-doensis Makino in diet effectively.This work provides a valuable reference for the positive bene-fits of applying VYM in poultry production,giving a viable solution for coping with the high-temperature climate of poultry farming.
万方数据
维普
