Study on the Effects and Mechanism of ART3 in Regulating Spermatogenesis in Mice
The study aimed to investigate the role and mechanism of ADP-ribosyl transferase 3(ART3)in spermatogenesis and deformation.In this study,21 healthy ICR male mices at 6-8 weeks were randomly selected and divided into control group,ART3 inhibitor 3-MBA treatment groups,with 3 replicates in each group.Testicular and epididymis tissues were collected for 12 h,24 h,48 h,5 d,7 d,and 14 d after testicular injection of 3-MBA in the treatment groups.Analysis of spermatogenesis in them showed that inhibition of ART3 resulted in testicular atrophy,decreased sperm density in the cauda epididymidis,and increased sperm deformity rate.Histological observation showed that the spermatogenic cells in the seminiferous tubules were gradually reduced and loosely arranged,and some of them were detached to the lumen;non-sperms components appeared in the caput and cauda epididymidis.The components were identified as round spermatids by immunofluorescence of acrosomal proteins.Further immunofluorescence showed that the inhibitor treatment reduced the level of Nectin-3,a cell adhesion protein between spermatids and sertoli cells,led to the apoptosis of spermatids and the expression of AKT signaling pathway-related proteins decreased.It is hypothesised that ART3 promotes round spermatids deformation by modulating spermatids-sertoli cells junctions and affecting the anti-apoptotic effect of AKT pathway.
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