The Effect of Adipose Mesenchymal Stem Cells on the Healing Process of Autologous Skin Transplantation in Bama Miniature Pigs
This study aims to use adipose derived mesenchymal stem cells(ADSCs)to intervene in an experimental animal model of autologous skin transplantation in small pigs,and observe their effects on the healing process of autologous skin transplantation.The animal model of autologous skin transplantation of 9 miniature pigs was established,and the miniature pigs were randomly divided into 3 groups:model group(Model),phosphate buffer group(PBS),and adipose mesenchymal stem cell group(ADSCs).Blood and skin tissue samples were collected before surgery and 7,14,21,and 28 days after surgery,respectively,and clinical changes of grafts and surrounding tissues were observed,photographed,and recorded.The effect of ADSCs on wound healing of mini pig autologous skin transplantation was comprehensively evaluated through skin graft apparent examination,histopathological examination,blood routine examination and serum biochemical examination,skin graft tissue oxidative stress,inflammation,regeneration and blood flow recovery.The researchers observed that the skin recovery speed of ADSCs group was better than that of the other two groups,and the ADSCs group basically returned to normal skin status 28 days after surgery.In terms of oxidative stress,SOD and GSH contents in ADSCs group were significantly increased(0.01＜P＜0.05),while MDA contents were significantly decreased(0.01＜P＜0.05).In terms of inflammation,the contents of TNF-α and IL-1β in ADSCs group were significantly decreased,and IL-10 was significantly increased on 7 and 14 days after surgery(P＜0.01).In terms of skin tissue regeneration,the expression levels of VEGF,bFGF,TGFβ3 and CD31 in ADSCs group were significantly increased(0.01＜P＜0.05).In terms of blood flow recovery,the percentage of blood perfusion of ADSCs during healing was significantly higher than that of the other two groups(0.01＜P＜0.05).To sum up,ADSCs can accelerate the recovery of skin tissue pathological damage,reduce oxidative stress reaction promote skin tissue regeneration and Angiogenesis in the miniature pig autologous skin transplantation animal model.The results indicated that ADSCs had the functions of anti-oxidation,anti-inflammation and promoting tissue regeneration,and had good therapeutic effect on autologous skin transplantation of miniature pigs.This experiment provided experimental support and scientific basis for the clinical application of stem cells in autologous skin transplantation and allogeneic skin transplantation.
small pigsadipose mesenchymal stem cellsautologous skin transplantationwound healing
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