Effects of Low Temperature on Adipose Tissue Morphology,Lipid Metabolism-Related Gene Expression and Enzyme Activities,and AMPK/PGC-1αPathway in Hezuo Pigs
This study aimed to investigate the effects of low temperature on adipose tissue mor-phology,lipid metabolism-related gene expression and enzyme activities,and AMPK/PGC-1αpathway in Hezuo pigs.Ten Hezuo pigs with similar body weight and good health condition at 75 days of age were selected and randomly divided into a control group((23±2)℃)and a low-temperature group((-15±2)℃),each with 5 pigs,after being reared for 7 d at room tempera-ture,for 15 d of the experimental period.At the end of the experiment,the adipose tissue was collected from the axilla and groin of the Hezuo pigs,and the morphology of the adipose tissue was observed by HE staining;the activity of lipid metabolism-related enzymes was detected by ELISA;the expression of adipose browning heat-producing genes and lipid metabolism-related genes was detected by fluorescence quantitative PCR;and the expression of the AMPK/PGC-1αpathway proteins was detected by Western blot.The results showed that,compared with the control group,the axillary and inguinal fat of the Hezuo pigs showed browning changes,the number of adipocytes became larger,and the cross-sectional area was reduced after the low-temperature treatment;LPL activity,IL-6 mRNA expression and AMPK,P-AMPK and PGC-1α protein expression were highly significantly increased in axillary and inguinal fat(P＜0.01),and LEP mRNA expression and FAS activity were highly significantly decreased(P＜0.01);UCP3 and HSP70 mRNA expression were significantly increased in axillary fat(P＜0.05),and PPARγ mRNA expression was significantly decreased(P＜0.05),AMPK and TNF-α mRNA expression was highly significantly increased(P＜0.01);AMPK and TNF-α mRNA expression was significantly increased(P＜0.05)in inguinal fat,UCP3 and HSP70 mRNA expression was highly significantly increased(P＜0.01),PPARγ mRNA expression was highly significantly decreased(P＜0.01);PGC-1α,ADPN,FAT mRNA expression and HSL activity were signifi-cantly increased(P＜0.05)in inguinal fat but not in axillary fat(P＞0.05)in Hezuo pigs after low-temperature treatment.In summary,the axillary fat and inguinal fat of Hezuo pigs under low temperatures underwent browning,and the area of adipocytes decreased,the number increased,the body heat production increased,and the key genes of lipid metabolism LEP,ADPN,PPARγ,FAT and the AMPK/PGC-1α pathway might be involved in the lipid metabo-lism of the organism.
国家科技期刊平台
NSTL
万方数据
