Effect of Erythropoietin on the Expression of Apoptotic Factor in Yak Renal Interstitial Fibroblasts
The purpose of this study was to investigate the effect of erythropoietin(EPO)on apoptosis factors in yak renal interstitial fibroblasts(RIFs).Firstly,the kidney tissues of 3 healthy yaks and cattle aged 4 years were collected respectively,immunohistochemistry and Western blot were used to detect the exact distribution of EPO and its expression in the kidneys of yaks and calves.Meanwhile,the yak RIFs were identified by primary culture,and different drug treatments were applied to regulate the EPO expression level in the RIFs,and the effects of EPO on the apoptosis-related genes Bax,Bcl-2,and PCNA at mRNA and protein expression levels were detected.Immunohistochemical results showed that EPO was positively expressed in collecting duct epithelial cells,renal tubular epithelial cells,and peritubular mesenchymal fibro-blast-like cells in the kidneys of both yaks and calves.qRT-PCR and Western blot results showed that the expression of EPO in the kidneys of yaks was significantly higher than that in the calves(P＜0.001).Subsequently,the EPO-generating RIFs were cultured in primary culture and the expression of EPO was regulated,and the results showed that the activation group significantly elevated the expression of Bcl-2 and PCNA and decreased the expression of Bax in the RIFs(P＜0.001).The EPO-inhibition group significantly decreased the expression of Bcl-2,PCNA and up-regulated the expression of Bax in the RIFs(P＜0.001).In conclusion,it was showed that EPO was positively expressed in collecting duct epithelial cells,renal tubular epithelial cells and peritubular mesenchymal fibroblast-like cells in the kidney of yak and calf,and the high expres-sion of EPO in the kidney of yak was able to promote the expression of Bcl-2 and PCNA and decrease the expression of Bax in the RIFs,which indicated that EPO could play an anti-apoptotic role in the kidney of yak.
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