Analysis of Serum Proteomics in Early Pregnancy of Yili Horses Based on 4D-DIA Technology
;The purpose of this article was to explore the serum proteomic characteristics of early pregnancy in Yili horses using proteomic techniques,screen for differential proteins,and analyze their roles in pregnancy.In this study,20 healthy Yili mares with the same age and similar weight were selected for artificial insemination after natural estrus.Pregnancy diagnosis was per-formed on the 13th day after ovulation in the mare.Three pregnant mares and three non pregnant mares were selected based on ultrasound results,and their serum samples were collected.Using 4D-DIA technology for protein quantitative and qualitative analysis of serum samples from Yili mares,screening differential proteins,and conducting bioinformatics analysis such as GO and KEGG.The results showed that a total of 823 proteins were identified,with a molecular weight range of 8-1 010 ku;Based on the threshold of absolute multiple of differences greater than 1.5 times and P＜0.05,53 proteins with significant differences were screened,of which 26 proteins were upregulated and 27 proteins were downregulated.Differential proteins mainly participated in biological processes such as cellular metabolism,biological regulation,metabolism,response to stimuli,cellular component organization,or biogenesis;The KEGG enrichment pathways included proteasomes,arginine biosynthesis,PI3K Akt signaling,ECM receptor interactions,and other pregnancy related pathways.Based on biological analysis such as GO and KEGG,six significantly different proteins were preliminarily screened for possible relationship with early pregnancy,and protein interaction network diagrams were drawn using them.The results showed that CTSS,MMP1,SERPINA5,and others were at key nodes.Six pregnancy specific proteins have been preliminarily screened in this study,providing basic data for screening preg-nancy specific proteins and molecular regulatory mechanisms in equine animals in the future.
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