Genomic Characterization of a Recombinant Strain of PRRSV-2 between Lineages 1.8 and 1.5
In order to investigate the epidemic variation of porcine reproductive and respiratory syndrome virus(PRRSV)strain in Tianjin area,a new epidemic strain of PRRSV(named TJ-C6)was isolated and identified from weaned piglets with severe respiratory symptoms by means of isolation and culture of porcine alveolar macrophages(PAMs),purification by limited dilution method and identification by IFA.The whole genome amplification and its molecular characteris-tics were analyzed.The results showed that the whole genome length of this strain was 15011 bp,and the nucleotide homology with NADC30 strain was the highest(91.7％),which was NADC30-like PRRSV(lineage 1.8).Nsp2 had 131 discontinuous deletion features("111+1+19aa").GP5 N-glycosylation site analysis showed that there were three glycosylation sites(N32,N43,N50).Recombination analysis showed that this strain was a recombinant PRRSV with NADC30-like(lineage 1.8)as the skeleton and NADC34-like(lineage 1.5),and its recombina-tion region was located in 12213-14628nt(ORF2a-ORF6).In this study,a recombinant PRRSV-2 strain of lineage 1.8 and lineage 1.5 was isolated and identified,which can provide ref-erence for the prevention and control of porcine reproductive and respiratory syndrome in Tianjin.
PRRSVisolation and identificationgenomic characterizationrecombination
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