Isolation and Molecular Characterization of Aichivirus C from Goats
Aichivirus C is a newly confirmed virus associated with caprine diarrhoea.The purpose of this study was to isolate goat Aichivirus C and analyze the genomic characteristics.Nine fecal samples and 13 tissue samples from a dead kid were collected from a goat farm with an outbreak of diarrhoea in Sichuan province,and used for testing the common pathogens associated with di-arrhoea.Virus was isolated from Aichivirus C positive samples using Vero cells and identified by PCR,indirect immunofluorescence and electron microscopy.The complete genomes of the iso-lates were amplified and analyzed.Results were as follows:8 fecal samples with diarrhoea and 8 tissue samples from a lamb were detected as positive for Aichivirus C by RT-PCR,and no other common diarrhoea-causing pathogens were detected in the above samples.Two Aichivirus C strains were successfully isolated from positive samples using Vero cells,and the virus titers were 106.5TCID50·mL-1 and 105.9TCID50·mL-1,respectively.The nearly full-length genomic se-quences of both strains were obtained,which shared 78.8％-97.4％nucleotide identity with the known goat Aichivirus C genomes from GenBank and 97.0％-97.4％nucleotide identity with the SWUN/F11/2019 isolate from China.Furthermore,the VP0,VP3 and VP1 genes were success-fully amplified from 5 clinical positive samples.Phylogenetic analysis based on the genome re-vealed that the two isolates were clustered with the SWUN/F11/2019 strain.Unique amino acid mutations were also revealed in the VP0 and VP1 genes.In summary,two strains of goat Aichi-virus C were successfully isolated and the nearly full-length genomic sequences were obtained.Phylogentic analysis demonstrated that these two strains are closely related to the SWUN/F11/2019 strain from China,with a unique molecular characteristics in the VP0 and VP1 genes.The results enrich the molecular genetic information of Aichivirus C from goats in China and laid the foundation for further studies on the pathogenicity and molecular biological characteristics of goat Aichivirus C.
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