Isolation,Identification and Drug Resistance Analysis of Klebsiella pneumoniae in Aborted Fetuses of Fox
In order to understand the etiology of abortion in foxes,the aborted fetuses in a fox farm in Hebei Province was analyzed by dissection,pathogen isolation and cultivation,16S rRNA sequencing,virulence gene detection,serotype detection,drug susceptibility analysisassay and pathogenicity analysis assay in this study.As a result,6 strains of Klebsiella pneumoniae were i-solated.Twelve virulence genes(wabG,fimH,ent,icuA,icuB,mrkA,ycf,ecp,uge,ure-A,iroB and ybtA)and three serotypes(K20,K54 and K57)were determined,and the K57 was the dominant serotype.Through K-B drug susceptibility testing,it showed that the isolated K.pneumoniae was moderately or highly sensitive to aminoglycosides,cephalosporins,quinolones,polypeptides and other antibacterial drugs,and multiple drug resistance to macrolides,tetracy-cline,penicillin and other antibacterial drugs was found.According to the pathogenicity analysis,the K.pneumoniae isolate had certain pathogenicity to mice,and the median lethal dose(LD50)was 1.2 × 107CFU·mL-1.In general,K.pneumoniae isolate showed certain pathogenicity,drug resistance,and carrying a variety of virulence genes in this study,and it might be one of the factors causing abortion of fox fetuses.The findings might provide hints for prevention and con-trol of fox fetal abortion.
foxKlebsiella pneumoniaevirulence genesolation and identification
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