疫苗接种是预防牛结节性皮肤病(lumpy skin disease,LSD)的重要措施,但是疫苗接种后诱导的体液免疫应答水平参差不齐,目前尚缺乏评价疫苗免疫效果的有效手段.本文使用R软件利用Meta分析对已发表的LSD免疫抗体监测结果进行统计,并进行亚组分析,综合评价免疫抗体监测的意义.通过计算机分别检索中国知网、万方数据知识服务平台、维普中文科技期刊数据库、PubMed、Web of science等中英文数据库,检索时限截止至2023年7月,收集LSD免疫抗体监测的相关文献,根据制定的文献纳入与排除标准,共纳入符合筛选条件的文献24篇,涉及羊痘疫苗的文献共有13篇,涉及LSD疫苗的文献共有13篇,其中2篇文献同时涉及羊痘疫苗和LSD疫苗,包含8 638例样本,其中阳性样本2 782份,结局指标为免疫抗体阳性率.Meta分析结果显示,羊痘疫苗的免疫抗体水平为41％(95％CI:27％～55％),LSD疫苗的免疫抗体水平为73％(95％CI:64％～86％),各文献间的同质性较低,两种疫苗之间无显著性差异(P=0.59),与羊痘疫苗接种(P=0.12)和LSD疫苗接种(P=0.077)相关的文献均提示未存在明显发表偏移;按养殖环境和检测方法分类进行亚组分析,不同养殖环境(P=0.84)和检测方法(P=0.40)均无显著性差异,两亚组均非异质性的主要来源.本文结果提示,使用免疫抗体监测方法评价免疫效果存在较大的不确定性,在实际工作中需要综合分析研判.
Meta Analysis of Immune Antibody Monitoring for Lumpy Skin Disease
Vaccination is an essential measure in preventing lumpy skin disease(LSD).However,the induction of humoral immune responses post-vaccination varies,and there is currently a lack of effective methods to evaluate vaccine immunogenicity.This study employed Meta-analysis using R software to statistically analyze published data on immune antibody monitoring for LSD,including subgroup analyses,to comprehensively assess the significance of immune antibody monitoring.A computer search was conducted in both Chinese and English databases,including CNKI,Wanfang Data,VIP,PubMed,and Web of Science.The search was up to July 2023,col-lecting relevant studies on immune antibody monitoring for LSD.Based on predetermined inclu-sion and exclusion criteria,a total of 24 articles were included.Among these,13 articles were re-lated to sheep/goat pox vaccine,and 13 articles were related to LSD vaccine.Two articles cov-ered both sheep/goat pox and lumpy skin disease vaccines,with a total of 8,638 samples,inclu-ding 2,782 positive samples.The outcome measure was the positive rate of immune antibodies.Meta-analysis results indicated an immune antibody level of 41％(95％CI:27％～55％)for sheep/goat pox vaccine and 73％(95％CI:64％～86％)for LSD vaccine.The homogeneity among studies was low,and there was no significant difference between the two vaccines(P=0.59).The literature related to sheep/goat pox vaccination(P=0.12)and LSD vaccination(P=0.077)both suggested no apparent publication bias.Subgroup analysis was based on immuniza-tion sites and detection methods,the mesults showed that there were no significant differences between different immunization sites(P=0.84)or detection methods(P=0.40),indicated non-heterogeneity in both subgroups.These findings suggest that there is significant uncertainty in e-valuating immune protection effectiveness using immune antibody monitoring methods,compre-hensive analysis and judgment will be necessitating in practical applications.
国家科技期刊平台
NSTL
万方数据
