基于宏基因组测序对中国北方地区生乳中嗜冷菌的群落结构研究
Study on the Community Structure of Psychrophilic Bacteria in Raw Milk from North Regions of China based on Metagenomic Sequencing
许文君 1郑楠 2王加启 2孟璐2
作者信息
- 1. 青岛农业大学食品科学与工程学院,青岛 266109;中国农业科学院北京畜牧兽医研究所农业农村部奶及奶制品质量安全控制重点实验室,北京 100193;中国农业科学院北京畜牧兽医研究所农业农村部奶产品质量安全风险评估实验室(北京),北京 100193
- 2. 中国农业科学院北京畜牧兽医研究所农业农村部奶及奶制品质量安全控制重点实验室,北京 100193;中国农业科学院北京畜牧兽医研究所农业农村部奶产品质量安全风险评估实验室(北京),北京 100193
- 折叠
摘要
本研究旨在利用宏基因组测序探究中国北方地区生乳中嗜冷菌群落结构的多样性.在冬季从中国内蒙古地区和北京地区的14个牧场共采集14份生乳样品,分离纯化可培养嗜冷菌和提取生乳中微生物总DNA,并利用16S rDNA测序和宏基因组测序结合的方法对生乳中嗜冷菌进行分析.结果发现,北京地区和内蒙古地区生乳中嗜冷菌的群落结构无显著性差异.两个地区生乳中优势嗜冷菌属均是假单胞杆菌属、寡养单胞菌属和短波单胞杆菌属.全局和概述图谱、氨基酸代谢和碳水化合物代谢是最主要的功能代谢途径,且嗜冷菌中含有多种抗生素抗性基因.综上,本研究利用传统培养和宏基因组测序准确测定了生乳中嗜冷菌群落的物种和功能信息,有助于了解生乳中嗜冷菌的生命活动及为嗜冷菌风险评估提供基础信息,促进中国北京及内蒙古牧场开展防控.
Abstract
The aim of this study was to investigate the community structure diversity of psychro-philic bacteria in raw milk in northern China by metagenomic sequencing.A total of 14 raw milk samples were collected from 14 pastures in Inner Mongolia and Beijing,China in winter.The cul-turable psychrophilic bacteria were isolated and purified,total microbial DNA in raw milk was extracted as well.Moreover,the psychrophilic bacteria in raw milk were analyzed by 16S rDNA sequencing and metagenomic sequencing,respectively.The results showed that no significant difference was found in community structure of psychrophilic bacteria in raw milk between Beijing and Inner Mongolia.Pseudomonas,Stenotrophomonas and Brevundimonas were the dominant psychrophiles bacteria in raw milk from the two regions.The global and overview profiles,amino acid metabolism and carbohydrate metabolism were the most important functional metabolic pathways,and psychrophilic bacteria contained a variety of antibiotic resistance genes.In conclu-sion,this study used traditional culture and metagenomic sequencing to accurately determine the species and functional information of the psychrophilic community in raw milk,which is helpful to understand the life activities of psychrophilic bacteria in raw milk and provide basic information for risk assessment of psychrophilic bacteria.Furthermore,it lays down foundational knowledge pivotal for the effective prevention and management of pasture-related issues in Beijing and Inner Mongolia,China.
关键词
生乳/嗜冷菌/宏基因组测序/地区/结构Key words
raw milk/psychrophilic bacteria/metagenomic sequencing/region/structure引用本文复制引用
基金项目
国家重点研发计划(2022YFD1301003)
农产品质量安全风险评估项目(GJFP20220304)
国家奶牛产业技术体系建设项目(CARS36)
中国农科院科技创新工程项目(ASTIP-IAS12)
出版年
2024
国家科技期刊平台
NSTL
万方数据