Effects of mdv1-miR-M4-5p Encoded by MDV on Proliferation and Apoptosis of MDCC-MSB1 Cells
The purpose of this experiment was to study the effects of mdv1-miR-M4-5p encoded by Marek's disease virus(MDV)on proliferation and apoptosis of MDCC-MSB1 cells.In this study,mdv1-miR-M4-5p mimics,inhibitors and their negative controls were transfected into MSB1 cells for 48 h.Real-time quantitative fluorescent PCR(qRT-PCR)was used to detect the transcription of mdv1-miR-M4-5p,TGF-β1,Smad2,caspase-9,caspase-3,cyt-c,cyclinD1,Bcl-2 et al other molecules related to proliferation and apoptosis in MDCC-MSB1 cells.Cell pro-liferation was detected by CCK-8 and cell cycle and apoptosis were detected by flow cytometry.The results showed that compared with the control group,the transcription levels of mdv1-miR-M4-5p,cyclinD1 and Bcl-2 in MDCC-MSB1 cells transfected with mdv1-miR-M4-5p mimics was significantly up-regulated.The transcription of TGF-β1,Smad2,cyt-c,caspase-9 and caspase-3 was down-regulated,the proliferation of MDCC-MSB1 cells was promoted,the the number of G1 phase cells were decreased,the S and G2 cells were increased,and the apoptosis rate was decreased.Compared with the control group,the results of the proliferation and apoptosis of MDCC-MSB1 cells and the expression of related molecules transfected with mdv1-miR-M4-5p inhibitor were opposite to those transfected with mdv1-miR-M4-5p mimics.The results showed that Mdv1-miR-M4-5p could promote the proliferation and inhibit the apoptosis of MDCC-MSB1 cells.This may be conducted by inhibiting the TGF-β1/Smad2 signaling pathway.
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