Horizontal Transfer of Drug Resistance Genes Carried by ICE_Prophage in Streptococcus suis SC124
This study aimed to investigate the transfer of antibiotic resistance genes(ARGs)car-ried by ICE_Prophage in Stre ptococcus suis.The whole genome sequencing of S.suis SC124 was performed using the Illumina MiSeq and Pacbio RSII platforms.The whole genome sequence of SC124 isolates was analyzed by online websites RAST,ICEberg 3.0,PH ASTER and NCBI data-base.MICs were determined by broth microdilution.The transferability of ICE_Prophage carry-ing ARGs in S.suis was investigated by conjugation.The present of circular intermediates was examined by inverse PCR.Competition was used to evaluate whether the transconjugant had fit-ness cost.The results showed that there was an ICE_Prophage(ICESssuSC124_ΦSsuSC124)car-rying tetracycline,macrolide and aminoglycoside resistance genes in S.suis SC124.ICESsuSC124 carrying tetracycline resistance genes was transferred at a frequency of 5.52 × 10-9,and no prophage transfer was found.Inverse PCR revealed that ICESsuSC124 formed a circular intermediate with a size of about 3.8 kb.Compared with S.suis BAA,the transconju-gant containing ICESsuSC124 did not have a competitive advantage under the action of drug-free pressure selection,and the transconjugant can produce a certain degree of fitness cost.In conclu-sion,the ICE_Prophage carrying tetracycline,macrolide and aminoglycoside resistance genes in S.suis SC124,in which ICESssuSC124 could be horizontally transferred,and the transconjugant could produce a certain degree of fitness cost.
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