Effects of Dandelion and Akebia Extract on Growth Performance,Intestinal Health and Relative Expression of Drug Transporter Genes in Weaned Rabbits
The objective of this experiment was to investigate the effects of dietary supplemented with dandelion and akebia extract on growth performance,diarrhea,intestinal morphology and structure,intestinal mucosa tight junction protein gene expression,drug transporter gene expres-sion in liver and intestine of weaned rabbits,and to explore whether the combination of dandelion and akebia extract can improve the bioavailability of Chinese medicine extract.A hundred and twenty 35-day-old weaned rabbits were randomly divided into 4 groups with 10 replicates per group and 3 rabbits per replicate.Four groups including control group(basic diet),dandelion group(basic diet+0.5％dandelion extract),akebia group(basic diet+0.5％akebia extract),and dandelion+akebia group(basic diet+0.5％dandelion extract+0.5％akebia extract).The trial lasted for 28 days.The results were showed as follows:1)At the first week of the experi-ment,compared with control group,the ADG in dandelion group and akebia group was signifi-cantly increased(P＜0.05),the value of F/G was significantly decreased(P＜0.05).2)The villus height of jejunum in dandelion group was significantly higher than that in akebia group(P＜0.05).3)The content of total flavonoids in jejunal mucosa of dandelion+akebia group was significantly higher than other groups(P＜0.05).4)The relative expression level of zonula oc-cluden 1(ZO-1)gene in ileum of group dandelion+akebia group was significantly higher than that of dandelion group and akebia group(P＜0.05).5)The relative expression levels of multi-drug resistance-associated protein 2(MRP2)and organic anion transporting polypeptide 2B1(OATP2B1)genes in jejunum of akebia group was significantly lower than those of control group(P＜0.05),and the relative expression level of OATP2B1 in jejunum of dandelion+akebia group was significantly lower than control group(P＜0.05).The relative expression of multidrug re-sistance-associated protein 3(MRP3)gene in ileum of dandelion group and akebia group was sig-nificantly lower than control group(P＜0.05).The relative expression levels of breast cancer re-sistance protein(BCRP)and MRP3 genes in liver of dandelion+akebia group were significantly lower than those of the control group(P＜0.05),and the relative expression levels of OATP2B1 gene in liver of akebia group and dandelion+akebia group were significantly lower than control group(P＜0.05).In conclusion,dietary supplementation of dandelion or akebia extract can im-prove the growth performance of weaned rabbits,combined supplementation of dandelion and akebia extract can increase the total flavonoid content in jejunum mucosa,and dietary supplemen-tation of dandelion and akebia extract can regulate the expression levels of drug transporter genes in liver and intestinal mucosa of weaned rabbits.
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