The aim of this study was to establish a rapid detection method of Schmallenberg virus(SBV).A real-time reverse transcription recombinase acid amplification(RT-RAA)was devel-oped with specific primers and probes based on of SBV S gene.The results showed that,through optimizing experimental conditions,the assay could detect SBV specifically within 8 minutes at 38 ℃.There was no cross-react with foot-and-mouth disease virus(FMDV),pestedes petites ru-minants virus(PPRV),African horse distemper virus(AHSV),Seneca valley virus(SVV),or vesicular stomatitis virus(VSV).The detection of limit(LOD)was 103 copies/reaction.At the same time,the co-efficient of variations in intra-and inter-assay were both less than 5%.The de-tection rate for the spinked positive samples and negative sheep samples was 100%,which was consistent with SBV RT-qPCR results.This study successfully established a fast,sensitive,and specific real-time RT-RAA assay for detection of SBV,which provided a new technical method for prevention and control of SBV infection.