Effects of FKBP5 on Function of Sheep Follicular Granulosa Cells
This study aimed to investigate the potential function of FKBP5 in ovine ovarian granulosa cells(GCs).GCs were isolated from the ovaries of twenty approximately 2-year-old sexually mature Altay sheep obtained from the Hualing slaughterhouse in Urumqi.The experiment was divided into 4 groups:GC(blank control),GC+NC(transfected with siRNA-NC),GC+siFKBP5(transfected with siRNA-FKBP5),and GC+siFKBP5+LH(treated with luteinizing hormone and transfected with siRNA-FKBP5).Immunofluorescence was used to detect the localization of FKBP5 protein in GCs.Its effects on cell proliferation and apoptosis were examined using EdU,Tunel assays,and Western Blot analysis.Its impacts on the AKT and ERK signaling pathways were assessed by Western blot,while the levels of E2 and P4 were measured by ELISA.FKBP5 was found to be expressed in the cytoplasm of GCs.Its expression increased with the increasing concentration of LH,reaching a peak at 5 IU·mL-1 LH after 4 h of treatment(P＜0.001).Knockdown of FKBP5 significantly inhibited cell proliferation,as evidenced by a reduction in PCNA protein expression(P＜0.001).It also significantly promoted cell apoptosis,with an increase in BAX expression and a decrease in Bcl-2 expression(P＜0.001).Additionally,FKBP5 knockdown markedly suppressed the activation of the AKT and ERK signaling pathways and the secretion of E2 and P4(P＜0.001).LH treatment partially mitigated the effects of FKBP5 knockdown on GCs(P＜0.05).The results indicate that FKBP5 influences the proliferation and apoptosis of ovine GCs,as well as the activation of the AKT and ERK signaling pathways and the secretion of E2 and P4.Its function is modulated byLH.These findings provide a theoretical basis for further research on the role of FKBP5 in ovine follicular development.
FKBP5granulosa cellscell proliferation and apoptosisAKT and ERK signaling pathwayssteroids
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