Isolation,Identification and Pathogenicity Analysis of a Pigeon Paramyxovirus-1 Strain
A strain of pigeon paramyxovirus-1(PPMV-1)was isolated from the dead pigeon tissue in Tianjin region.To provide scientific basis for the development of PPMV-1 vaccines,the genetic evolution,biological characteristics,and pathogenicity of the virus were analyzed.The pathogens were identified by RT-PCR,virus isolation,purification by chicken embryos,genetic evolution analysis,pathogenicity analysis,and animal regression tests.A strain of PPMV-1 was isolated from the dead pigeons and named Pigeon/TJ/CH/020/2020(TJ20).Sequence analysis and homology analysis of the virus F gene showed that the strain belonged to sub-genotype Ⅵ.2.1.1.2.2(Ⅵk),and the amino acid residues of the cleavage site were 112R-R-Q-K-R-F117,which were consistent with the molecular characteristics of NDV virulent strains.Hemagglutination inhibition revealed a noticeable difference between TJ20 and LaSota.The values of TCID50,EID50,MDT and ICPI were 10-8.64·0.1 mL-1,10-8.38·0.1 mL-1,62 h,1.19.The animal infection experiment found that pigeons showed clinical symptoms on 4 dpc(day 4 post-challenge).The incidence rate and mortality were 100％,and the cloacal detoxification rate could reach 100％on 5 dpc.TJ20 was highly pathogenic to pigeons.This study successfully isolated a highly pathogenic sub-genotype Ⅵ.2.1.1.2.2(Ⅵ k)TJ20 strain from samples of dead pigeons,providing important biological materials for the research and development of vaccine of PPMV-1 in the future.
pigeon paramyxovirus-1isolation and identificationsub-genotype Ⅵ.2.1.1.2.2(Ⅵk)pathogenicity
万方数据
维普
