陈皮精油对脂多糖诱导的RAW264.7细胞炎症的干预作用

Intervention of Essential Oils from Citrus reticulata Blanco on Lipopolysaccharide-induced Inflammation in RAW264.7 Cells

刘玲 ¹史万玉 ¹李秀梅 ²王明华 ¹翟向和 ¹周炜炜²

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作者信息

1. 河北农业大学中兽医学院,保定 071000
2. 中国农业科学院饲料研究所农业农村部饲料生物技术重点实验室,北京 100081
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摘要

基于脂多糖(lipopolysaccharide,LPS)诱导的RAW264.7细胞模型研究陈皮精油抗炎作用.通过Griess法检测一氧化氮(nitric oxide,NO)分泌水平,采用Real-time PCR法检测细胞中肿瘤坏死因子(tumor necrosis factor α,TNF-α)、白细胞介素 6(interleukin-6,IL-6)、白细胞介素 1β(interleukin-1β,JL-1β)和诱导型一氧化氮合酶(inducible nitric oxide synthase,iNOS)、环氧化酶 2(cyclooxygenase 2,COX-2)mRNA 的表达水平.结果显示,陈皮精油显著降低LPS诱导的RAW264.7细胞释放NO的含量,抑制iNOS、COX-2、IL-1β、IL-6和TNF-α mRNA的表达.综上所述,陈皮精油通过抑制炎症因子的分泌和表达,有效缓解由LPS诱导RAW264.7细胞炎症反应.

Abstract

The anti-inflammatory effect of essential oils from Citrus reticulata Blanco was investigated based on lipopolysaccharide(LPS)-induced RAW264.7 cell model.The level of Nitric oxide(NO)secretion was detected by Griess method,and the mRNA expression levels of tumor necrosis factor(TNF-α),interleukin-6(IL-6),interleukin-1β(IL-1β),inducible nitric oxide synthase(iNOS)and cyclooxygenase 2(COX-2)were determined by real-time PCR method.The results showed that essential oils from Citrus reticulata Blanco significantly decreased the level of NO released from LPS-induced RAW264.7 cells and inhibited the expression of iNOS,COX-2,IL-1β,IL-6 and TNF-α mRNA.In conclusion,essential oils from Citrus reticulata Blanco can inhibit the secretion and expression of inflammatory factors,and thus alleviate the inflammatory response induced by LPS in RAW264.7 cells effectively.

关键词

陈皮/精油/抗炎/LPS/RAW264.7细胞

Key words

Citrus reticulata Blanco/essential oils/anti-inflammatory/LPS/RAW264.7 cell

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基金项目

农业农村部饲料生物技术重点实验室开放课题(KLFB-FRI-202104)

中国农科院创新工程联合攻关重大科研任务(CAAS-ZDRW202111)

出版年

2024

畜牧兽医学报

中国畜牧兽医学会

畜牧兽医学报

CSTPCDCSCD北大核心

影响因子：0.729

ISSN：0366-6964

参考文献量31

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