Intervention of Essential Oils from Citrus reticulata Blanco on Lipopolysaccharide-induced Inflammation in RAW264.7 Cells
The anti-inflammatory effect of essential oils from Citrus reticulata Blanco was investigated based on lipopolysaccharide(LPS)-induced RAW264.7 cell model.The level of Nitric oxide(NO)secretion was detected by Griess method,and the mRNA expression levels of tumor necrosis factor(TNF-α),interleukin-6(IL-6),interleukin-1β(IL-1β),inducible nitric oxide synthase(iNOS)and cyclooxygenase 2(COX-2)were determined by real-time PCR method.The results showed that essential oils from Citrus reticulata Blanco significantly decreased the level of NO released from LPS-induced RAW264.7 cells and inhibited the expression of iNOS,COX-2,IL-1β,IL-6 and TNF-α mRNA.In conclusion,essential oils from Citrus reticulata Blanco can inhibit the secretion and expression of inflammatory factors,and thus alleviate the inflammatory response induced by LPS in RAW264.7 cells effectively.
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