Limosilactobacillus reuteri Alleviates Intestinal Ischemia/Reperfusion Injury in Rats by Inhibiting the ROS Dependent NLRP3 Inflammatory Pathway
The aim of this study is to explore the mechanism of Limosilactobacillus reuteri(LR)improving intestinal ischemia-reperfusion injury(IR)using ROS/NLRP3 inflammasome as a starting point.The IR cell model was constructed using hypoxia/reoxygenation methods,and the rat IR model was constructed using microarterial clamping/perfusion methods.The cell experiment was divided into four groups:control group,IR group,LR pre-treatment group(LR+IR group),and LR group.The animal experiment was divided into three groups(10 rats in each group):sham surgery group(S group),IR group,and LR gavage treatment group(LR+IR group).The level of ROS,the expression of NLRP3 inflammasome related proteins(NLRP3,ASC,Caspase p10,and GSDMD),the levels of inflammation-related indicators,and the content of oxidative stress related indicators in each group were detected.Histopathological evaluation was also performed.The results showed that:1)LR attenuates IR induced oxidative stress and NLRP3 inflammasome activation.2)LR alleviates NLRP3 inflammasome activation by attenuating ROS expression.3)LR could alleviate intestinal injury and the expression of inflammatory factors induced by IR.In summary,LR can alleviate intestinal IR and oxidative stress in rats,and its mechanism of action is related to the inhibition of ROS dependent NLRP3 inflammasome,providing experimental basis for the veterinary clinical treatment of LR in intestinal related diseases.
万方数据
维普
