Inhibitory Effect of Resveratrol on Rotavirus-infected Porcine Intestinal Epithelial Cells IPEC-J2
The aim of this study was to investigate the antiviral effect of resveratrol on porcine rotavirus(PoRV)infected porcine intestinal epithelial cells(IPEC-J2).In this experiment,IPEC-J2 cells were used as research object.Firstly,the effect of different concentration of resveratrol on the cell viability of IPEC-J2 was determined.Then experimental groups including negative control group,PoRV-infected group and PoRV-infected group with the addition of resveratrol at different time points were set up depending on whether the IPEC-J2 cells were treated with PoRV or resveratrol(100 μmol·L-1).The replication and proliferation of PoRV in IPEC-J2 cells were detected by real-time fluorescence quantitative PCR,viral titer assay and Western blot.The results showed that:1)The addition of resveratrol(100 μmol·L-1)significantly inhibited PoRV replication and infection at the late and full phases of viral infection of PoRV infection with IPEC-J2(P＜0.05);2)Compared with the PoRV-infected group,the addition of resveratrol(100 μmol·L-1)during the full phase of PoRV-infected IPEC-J2 extremely significantly suppressed the levels of inflammatory cytokines IL-1β,IL-10,TNF-α and IFN-β in the supernatant fluid of IPEC-J2 cells(P＜0.01),while significantly suppressed the IL-10 and IFN-β levels during the late phase(P＜0.05);Extremely significantly suppressed the relative expression of immune-related factors MDA5,RIG-I,TRIF,MAVS,LGALS9,EIF2AK2,IRF9 and IFI44L in IPEC-J2 cells(P＜0.01)during late and full phases;Extremely significantly suppressed the relative mRNA expression of variable splicing factors SRPK1,SRPK2,HNRNPC and HNRNPR in IPEC-J2 cells(P＜0.01)during late and full phases;3)Compared with the negative control group,PoRV infection significantly promoted exon jumps in exon 5 of LGALS9 and exon 2 of EF2AK2 in IPEC-J2 cells,while the addition of resveratrol(100 μmol·L-1)significantly inhibited the exon skipping of target genes in the late and full phases of PoRV infection IPEC-J2 cells.In conclusion,this study confirmed that the addition of 100 μmol·L-1 resveratrol could inhibit the infection and replication of PoRV in IPEC-J2 cells and play a role in the late phases of PoRV replication,which can provide a new basis and reference for the prevention and treatment of viral diarrhea in piglets.
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