摘要
干细胞在疾病发生、细胞治疗、药物筛选和临床应用等方面具有巨大价值.自日本科学家Shinya Ya-manaka发现诱导多能干细胞以来,相关的研究立即成为研究热点并持续至今.小鼠干细胞的研究为其它动物的相关研究开辟了道路,但在药物筛选和疾病治疗等方面有很大局限性.非人灵长类与人类具有更高的基因同源性,在生理、病理、生殖、药理、神经等多方面与人类高度相似,是研究人类疾病和开发人用药物的理想动物模型.非人灵长类研究也是新药在进入临床实验前必须的一个环节.非人灵长类干细胞的研究在疾病的机理研究和药物开发中越发重要,同时避免了伦理问题.本文从体外培养系统的优化和诱导分化的方法两个方面对非人灵长类多能干细胞的相关研究进行了综述,以期为非人灵长类干细胞进一步深入研究提供参考.
Abstract
Stem cells have great value in disease occurrence,cell therapy,drug screening,and clinical applications.Since Shinya Yamanaka,a Japanese scientist,discovered induced pluripo-tent stem cells(iPSCs),the relevant research has become a research hotspot and up to the pres-ent.The study of mouse stem cells has opened up a path for the related research in other ani-mals,but there are significant limitations in drug screening and disease treatment.Non-human primates have higher genetic homology with humans and are highly similar to humans in physiol-ogy,pathology,reproduction,pharmacology,nerves,and other aspects.They are ideal animal models for studying human diseases and developing drugs for human.Research on non-human primates is also a necessary step for a new drugs before entering clinical trials.The study of non-human primate stem cells is becoming important in the mechanism of diseases and drug develop-ment,while avoiding ethical issues.This article reviews the related research on non-human pri-mate pluripotent stem cells including optimization of in vitro culture systems and methods of in-ducing differentiation,aiming to provide reference for further in-depth research on non-human primate stem cells.
维普
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