首页|单细胞转录组学技术及其在寄生虫研究中的应用进展

单细胞转录组学技术及其在寄生虫研究中的应用进展

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单细胞转录组代表了某一时刻单个细胞内所有mRNA总表达量,其表达量反映该细胞的总体特征.通过单细胞转录组测序(single cell RNA sequencing,scRNA-seq)技术对单个细胞的全部RNA进行逆转录、扩增和测序,测序结果中包含的大量信息有可能重塑对发育生物学、基因调控以及健康和疾病中细胞异质性的理解.随着科学技术的不断发展,测序技术也在不断进步,scRNA-seq全方位、高通量以及高分辨率的特点可构建更细致、全面和精准的单细胞图谱.本文综述了常用scRNA-seq方法及其数据分析主要流程和单细胞转录组学技术在寄生虫学与寄生虫病领域研究中的应用,以期为该领域相关研究提供参考资料.
Progress on Single-cell Transcriptomics Technology and Its Applications in Research on Parasites
The single cell transcriptome represents the total expression of all mRNA in a single cell at a certain time,and its expression level reflects the overall characteristics of the cell.The entire RNA of a single cell is reversely transcribed,amplified and sequenced by single cell RNA sequencing(scRNA-seq).The extensive information contained in the sequencing results has the potential to reshape the understanding of developmental biology,gene regulation and cellular het-erogeneity in health and disease.With the continuous development of science and technology,se-quencing technologies are also constantly improving.The omni-directional,high-throughput and high-resolution characteristics of scRNA-seq can build a more detailed,comprehensive and accu-rate single cell map.This paper summarizes the commonly used scRNA-seq methods,the main processes of data analysis,and the application of single cell transcriptome technology in the field of parasitology and parasitic diseases,in order to provide reference materials for related research in this field.

scRNA-seqtranscriptomedata analysisparasitic diseases

周蜓蜓、李立、吴燕涛、逯文颖、付宝权、殷宏、贾万忠、闫鸿斌

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中国农业科学院兰州兽医研究所,兰州大学动物医学与生物安全学院,动物疫病防控全国重点实验室,国家动物包虫病专业实验室,甘肃省病原生物学基础学科研究中心,甘肃省动物寄生虫病重点实验室,农业农村部动物病原生物学重点实验室,农业农村部反刍动物重大疫病防控重点实验室(西部),兰州 730000

江苏省动物重要疫病与人兽共患病防控协同创新中心,扬州 225009

单细胞测序 转录组 数据分析 寄生虫病

国家重点研发计划甘肃省科技重大专项甘肃省科技重大专项甘肃省在站博士后专项国家肉牛牦牛产业技术体系项目兰州大学中央高校基本科研业务费专项动物疫病防控全国重点实验室重大成果培育项目中国农业科学院创新工程中国农业科学院创新工程

2022YFD180020023ZDNA00722ZD6NA00123JRRA558CARS-37lzujbky-2024-ydyl02SKLADCP2023HP05CAASTIP-2024-5CAAS-ASTIP-2021-LVRI

2024

10.11843/j.issn.0366-6964.2024.10.005

畜牧兽医学报
中国畜牧兽医学会

畜牧兽医学报

CSTPCD北大核心
影响因子：0.729
ISSN：0366-6964
年,卷(期)：2024.55(10)