The single cell transcriptome represents the total expression of all mRNA in a single cell at a certain time,and its expression level reflects the overall characteristics of the cell.The entire RNA of a single cell is reversely transcribed,amplified and sequenced by single cell RNA sequencing(scRNA-seq).The extensive information contained in the sequencing results has the potential to reshape the understanding of developmental biology,gene regulation and cellular het-erogeneity in health and disease.With the continuous development of science and technology,se-quencing technologies are also constantly improving.The omni-directional,high-throughput and high-resolution characteristics of scRNA-seq can build a more detailed,comprehensive and accu-rate single cell map.This paper summarizes the commonly used scRNA-seq methods,the main processes of data analysis,and the application of single cell transcriptome technology in the field of parasitology and parasitic diseases,in order to provide reference materials for related research in this field.
维普
万方数据