Changes in Gut Microbiota after ZBED 6 Knockout were Analyzed Based on 16S rRNA Sequencing
The aim of this study was to investigate the association between changes in gut micro-biota and muscle phenotype in ZBED 6-knockout Bama pigs.In this study,5-month-old wild-type and ZBED 6-knockout Guangxi Bama mini-pigs were divided into 3 groups based on sex and genotypes:wild-type boars compared with wild-type sows(male WT:female WT),wild-type sows compared with knock-out sows(female WT:female Ko),and wild-type boars compared with knock-out boars(male WT:male Ko).The microbial composition of each intestinal seg-ment was analyzed by high-throughput sequencing of 16S rRNA gene.The results showed that:1)The community diversity of large intestine in Bama pigs was significantly higher than that of small intestine;2)Compared with female wild-type pigs,male wild-type pigs have higher lean meat percentage.Turicibacter in ileum,Streptococcus in cecum and Peptostreptococcaceae in rectum were found to be concentrated in male wild-type pigs,while Lactobacillus in ileum was found to be concentrated in female wild-type pigs;3)After knockout of ZBED6,muscle growth increased in pigs.The content of Lactobacillus in ileum of sows was significantly decreased and SMB53 in cecum was significantly increased.After knockout of ZBDE6,Prevotella and Rumi-nococcus increased significantly in boar rectum,and SMB53 increased significantly in cecum.The results suggest that the differences in abundance of Turicibacter,Streptococcus,Peptostrepto-coccaceae and Lactobacillus in the gut may affect energy metabolism and promote muscle growth in male wild-type pigs;After ZBED6 knockout,an increase abundance in SMB53 in the cecum of pigs may be a factor contributing to muscle gain.
万方数据
维普
