首页|基于SNP芯片信息分析西藏四个本地牛种的血统组成

基于SNP芯片信息分析西藏四个本地牛种的血统组成

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旨在探究西藏4个本地牛种的遗传结构和血统组成.本研究以22头高原荷斯坦牛与20头高原娟姗牛以及45头美国婆罗门牛、46头云南瘤牛和11头婆罗门牛为参照标准品种,待测样品为4个西藏本地品种的177头牛.通过Illumina GGP100K芯片杂交获取所有待测个体的SNPs数据,利用MEGA X和Admixture等软件对SNPs进行遗传结构与血统组成分析.结果表明,西藏牛较其他西藏本地牛种与高原荷斯坦牛和高原娟姗牛有更近的遗传关系;部分樟木牛和阿沛甲咂牛将西藏牛分为两个大群;日喀则驼峰牛与云南瘤牛遗传关系更近.在日喀则驼峰牛群体中,高原牛血统比例超过90%,在阿沛甲咂牛与西藏牛群体中,高原牛血统也超过了 50%,仅在樟木牛群体中,高原牛血统比例为33.15%.其次,在樟木牛群体中,瘤牛血统占比高达18.27%,在阿沛甲咂牛、西藏牛与日喀则驼峰牛群体中,瘤牛血统较少(5%).在阿沛甲咂牛群体内有高达35.94%的普通牛血统,在樟木牛群体内,奶牛血统高达48.58%.本研究对西藏本地牛种今后的杂交扩繁和横交固定提供了重要指导作用,也为阿沛甲咂牛与樟木牛两个濒危品种的资源保护和利用提供了理论参考.
Study on the Genetic Composition of Four Local Tibetan Cattle Breeds Based on SNP Chip Analysis
The study aimed to explore the genetic structure and bloodline composition of 4 local cattle breedings in Tibet.In this study,22 plateau Holstein cattle,20 plateau Jersey cattle,45 American Brahmin cattle,46 Yunnan Zebu and 11 Brahmen cattle as reference breeds,the sam-ples to be tested were 177 cattle of 4 local breeds in Tibet.SNPs data were obtained by Illumina GGP100K chip hybridization,and the genetic structure and bloodline composition of SNPs were analyzed by MEGA X and Admixture.The results showed that Tibetan cattle had a closer genetic relationship with plateau Holstein cattle and plateau Jersey cattle than other local cattle breeds in Tibet.Some Zhangmu cattle and Apeijia cattle divided Tibetan cattle into two large subpopula-tions;There was a close genetic relationship between Shigatse hump cattle and Yunnan Zebu.In the Shigatse hump cattle population,the proportion of plateau cattle bloodline was more than 90%,in the Apaijia cattle population and Tibet cattle population,the proportion of plateau cattle bloodline was more than 50%,only in the Zhangmu cattle population,the proportion of plateau cattle bloodline was 33.15%.Secondly,bos indicus bloodline accounted for 18.27%in Zhangmu cattle population,while bos indicus bloodline was less(5%)in Apaijiza cattle,Tibet cattle and Shigatse hump cattle population.There were 35.94%dairy cattle bloodline in Apaijia population and 48.58%dairy cattle bloodline in Zhangmu population.This study provided important guid-ance for the future cross-breeding and cross-fixing of the native cattle species in Tibet,and also provided theoretical reference for the conservation and utilization of the resources of the two en-dangered breeds of Apaijiza cattle and Zhangmu cattle.

Apejiaza cattleZhangmu cattleTibetan cattleShigatse hump cattleSNPs datagenetic structurebloodline composition

马龙刚、刘楠、尼玛群宗、王欣、陆健、毛华明、陈功、旦增欧珠、拉巴次仁、张楠、于福清、王雅春

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中国农业大学动物科学技术学院,北京 100193

西藏自治区动物疫病预防控制中心,拉萨 850009

北京奶牛中心,北京 100192

全国畜牧总站,北京 100125

云南农业大学动物科学技术学院,昆明 650201

北京交通大学生命科学与生物工程研究院,北京 100044

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阿沛甲咂牛 樟木牛 西藏牛 日喀则驼峰牛 SNPs数据 遗传结构 血统组成

西藏自治区重点研发计划国家现代农业产业技术体系西藏自治区区域协同创新专项

CGZH2023000257CARS-36QYXTZX-LS2021-01

2024

畜牧兽医学报
中国畜牧兽医学会

畜牧兽医学报

CSTPCD北大核心
影响因子:0.729
ISSN:0366-6964
年,卷(期):2024.55(10)