Study on the Genetic Composition of Four Local Tibetan Cattle Breeds Based on SNP Chip Analysis
The study aimed to explore the genetic structure and bloodline composition of 4 local cattle breedings in Tibet.In this study,22 plateau Holstein cattle,20 plateau Jersey cattle,45 American Brahmin cattle,46 Yunnan Zebu and 11 Brahmen cattle as reference breeds,the sam-ples to be tested were 177 cattle of 4 local breeds in Tibet.SNPs data were obtained by Illumina GGP100K chip hybridization,and the genetic structure and bloodline composition of SNPs were analyzed by MEGA X and Admixture.The results showed that Tibetan cattle had a closer genetic relationship with plateau Holstein cattle and plateau Jersey cattle than other local cattle breeds in Tibet.Some Zhangmu cattle and Apeijia cattle divided Tibetan cattle into two large subpopula-tions;There was a close genetic relationship between Shigatse hump cattle and Yunnan Zebu.In the Shigatse hump cattle population,the proportion of plateau cattle bloodline was more than 90%,in the Apaijia cattle population and Tibet cattle population,the proportion of plateau cattle bloodline was more than 50%,only in the Zhangmu cattle population,the proportion of plateau cattle bloodline was 33.15%.Secondly,bos indicus bloodline accounted for 18.27%in Zhangmu cattle population,while bos indicus bloodline was less(5%)in Apaijiza cattle,Tibet cattle and Shigatse hump cattle population.There were 35.94%dairy cattle bloodline in Apaijia population and 48.58%dairy cattle bloodline in Zhangmu population.This study provided important guid-ance for the future cross-breeding and cross-fixing of the native cattle species in Tibet,and also provided theoretical reference for the conservation and utilization of the resources of the two en-dangered breeds of Apaijiza cattle and Zhangmu cattle.