Study on the Function of PPP5C Gene in Regulating the Proliferation and Differentiation of Bovine Adipocytes
The aim of this study was to investigate the role of PPP5C gene in the proliferation and differentiation of bovine adipocytes.Loss-of-function and gain-of-function experiments of PPP5C gene were performed in precursor adipocytes of Guyuan cattle.mRNA expression levels of marker genes and cell proliferation viability were detected using qRT-PCR,EdU,CCK-8 and flow cytometry.The results of TG assay,Oil Red O,BODIPY and Nile red staining provided phenotypic data support of lipid droplets accumulation during adipocytes differentiation.The re-sults showed that,compared with the control group,interfering with the PPP5C gene signifi-cantly up-regulated the mRNA expression of proliferation marker genes(CDK1,CDK2,and PCNA)(P＜0.01),and cell viability was significantly increased(P＜0.01),which facilitated the transition of bovine precursor adipocytes from the S-phase to the G2-phase(P＜0.01).The results also suggested that PPP5C might also play a negative regulatory role in bovine precursor adipose differentiation.This was mainly manifested in the significant up-regulation of mRNA ex-pression of lipogenic marker genes(PPARγ,C/EBPɑ,and FABP4)(P＜0.01)and the signifi-cant increase of TG content in adipocytes(P＜0.01)after interfering with the PPP5C gene.Meanwhile,the results of cellular phenotypic data of Oil Red O,BODIPY and Nilered staining suggested that interfering with PPP5C promoted bovine precursor adipocyte differentiation and increased lipid droplet accumulation.The results of functional acquisition experiments with the PPP5C gene were just the opposite.In summary,interfering with the PPP5C gene promotes bovine precursor adipocyte proliferation and differentiation,and overexpression of the PPP5C gene inhibits bovine precursor adipocyte proliferation and differentiation,suggesting that the PPP5C gene may play a role as a negative regulator in bovine precursor adipocyte proliferation and differentiation.This study provides basic data for further investigation of the molecular mechanism of PPP5C gene in regulating bovine fat deposition.
万方数据
维普
