Mitochondrial Genome Assembly and Phylogenetic Analysis of the Jiuyishan Rabbit
The aim of the study was to assemble the mitochondrial genome of the Jiuyishan rabbit and to investigate its phylogenetic evolution and taxonomic position.High-throughput sequencing technology was employed to perform whole-genome sequencing on ear tissues from 20 Jiuyishan rabbits at 150 days of age.Bioinformatics software was employed for the assembly of the mito-chondrial genome,followed by conducting sequence analysis,gene prediction and annotation of the obtained mitochondrial genome.Subsequently,multiple sequence alignment and phylogenetic analysis were conducted based on the published mitochondrial genome sequences of rabbits,hares,economically important animals and model animals.Polymorphic sites within the study population were identified.The complete mitochondrial genome sequence of the Jiuyishan rabbit was successfully obtained,the length was 17 306 bp.The nucleotide composition and gene ar-rangement of the mitochondrial genome were found to be largely consistent with those reported for 5 previously published domestic rabbit breeds.Phylogenetic analysis based on the D-loop re-gion indicated a closer evolutionary relationship between the Jiuyishan rabbit and the Fujian Yellow rabbit,while Jiuyishan rabbit was more distantly related to the European rabbit,Yimeng Wool rabbit,Chuanbai Rex rabbit and New Zealand White rabbit.Interspecific phylogenetic analysis involving 14 wild rabbit species and 11 economic or model animal species demonstrated that the Jiuyishan rabbit shared a closer phylogenetic relationship with the European wild rabbit,H ainan rabbit,and the White-booted rabbit but was further with others.A total of 12 mutation sites were identified in the conserved regions of the mitochondrial genome of the study popula-tion,with 3 mutations having allele frequencies greater than 0.8,indicating polymorphism.This study has successfully assembled the first complete mitochondrial genome sequence of the Jiuyishan rabbit,clarifying its phylogenetic relationships and taxonomic status in comparison to other domestic rabbit breeds,wild rabbits and common economic or model animals.This work provides foundational data for the utilization of genetic resources and the breeding of the Jiuyishan rabbit.
万方数据
维普
