Effect of Sericin on Cryopreservation of Semen of Chengde Hornless Black Goat
The aim of this study was to explore the effect of sericin on cryopreservation of semen of Chengde hornless black goat.Semen samples of 3 healthy Chengde hornless black goats were col-lected by the sham vaginal method and randomly divided into 6 groups:control group and experimental groups with different sericin concentrations(0.2％,0.4％,0.6％,0.8％,1％)added to the Op-tiXcell 2 dilution.Each group was set up with 5 replicates.Sperm motility,acrosome integrity rate,plasma membrane integrity rate,total antioxidant capacity(T-AOC),glutathione peroxi-dase(GSH-Px),superoxide dismutase(SOD),catalase(CAT)activity,malondialdehyde(MDA)content,mitochondrial membrane potential(MMP)and ATP content were detected af-ter freezing and thawing.The results showed that the addition of different concentrations of seri-cin to the semen frozen dilution of Chengde hornless black goat could improve the quality of sperm af-ter freezing and thawing.Compared with other concentrations,the addition of 0.6％sericin significantly increased sperm motility,acrosome integrity rate,plasma membrane integrity rate,mitochondrial mem-brane potential and ATP content after sperm freezing and thawing,and increased the activities of antioxi-dant enzymes T-AOC,GSH-Px,SOD and CAT to(13.61±0.39)mmol g-1,(26.52±1.30)U·mg-1,(165.45±11.10)U·mg-1 and(13.76±0.30)U·mg-1,respectively.Meanwhile,the MDA value was reduced to(3.15±0.20)μmol·mg-1.In summary,sericin at different concen-trations can improve the quality of frozen semen of Chengde hornless black goat,and the most suitable concentration is 0.6％.
sericinChengde hornless black goatantioxidantssemen freezing
万方数据
维普
