The aim of this study was to explore the effect of sericin on cryopreservation of semen of Chengde hornless black goat.Semen samples of 3 healthy Chengde hornless black goats were col-lected by the sham vaginal method and randomly divided into 6 groups:control group and experimental groups with different sericin concentrations(0.2％,0.4％,0.6％,0.8％,1％)added to the Op-tiXcell 2 dilution.Each group was set up with 5 replicates.Sperm motility,acrosome integrity rate,plasma membrane integrity rate,total antioxidant capacity(T-AOC),glutathione peroxi-dase(GSH-Px),superoxide dismutase(SOD),catalase(CAT)activity,malondialdehyde(MDA)content,mitochondrial membrane potential(MMP)and ATP content were detected af-ter freezing and thawing.The results showed that the addition of different concentrations of seri-cin to the semen frozen dilution of Chengde hornless black goat could improve the quality of sperm af-ter freezing and thawing.Compared with other concentrations,the addition of 0.6％sericin significantly increased sperm motility,acrosome integrity rate,plasma membrane integrity rate,mitochondrial mem-brane potential and ATP content after sperm freezing and thawing,and increased the activities of antioxi-dant enzymes T-AOC,GSH-Px,SOD and CAT to(13.61±0.39)mmol g-1,(26.52±1.30)U·mg-1,(165.45±11.10)U·mg-1 and(13.76±0.30)U·mg-1,respectively.Meanwhile,the MDA value was reduced to(3.15±0.20)μmol·mg-1.In summary,sericin at different concen-trations can improve the quality of frozen semen of Chengde hornless black goat,and the most suitable concentration is 0.6％.
sericinChengde hornless black goatantioxidantssemen freezing
维普
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