Pathogenicity of a Newly Akabane Virus on Goats from Bamboo Rat
In order to determine the pathogenicity of Akabane virus(AKAV)from bamboo rat in goats,GXLCH16-70 strain was used to infect goats via intracranial(IC),intravenous(Ⅳ)and subcutaneous(SC)inoculation.The clinical manifestations,viremia,pathological changes,his-topathology,and pathogenic distribution in tissue or organ of goats were observed.The 4th to 9th day post infection(dpi),goats in the IC group showed obvious neurological symptoms such as intermittent spasm,convulsion,while goats in the Ⅳ and SC groups showed symptoms as such diarrhea and increased secretions in the eyes at the 3rd to 8th dpi.All goats infected by GX-LCH16-70 developed viremia at the 2nd to 10th dpi,and produced AKAV neutralizing antibodies at the 8th dpi.The antibodies continued to exist up to the 21st dpi.Autopsy in the intracranial group showed non-suppurative encephalomyelitis of the brain,pulmonary congestion and hemor-rhage,marginal hemorrhagic spots of the spleen and other pathological changes.Histopathologi-cal examinations on the IC group showed the multiple layers of inflammatory cells infiltrating to the meninges and cortical blood vessels,forming a"vascular sheath"like structure.Pathogen de-tection by fluorescence quantitative RT-PCR(qRT-PCR)showed that AKAV-S RNA fragments were detected in almost all regions of the brain in the IC group,and in some brain tissue and spinal cord in the Ⅳ group and SC group.The results showed that AKAV GXLCH16-70 strain from bamboo rat causes systemic lesions of multiple organs and tissues of goats and induce encephalomyelitis.
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维普
