Porcine encephalomyocarditis is a viral zoonotic disease caused by encephalomyocarditis virus and characterized by porcine encephalitis and myocarditis,which can infect a variety of mammals.Currently,there is no effective vaccine in China,so developing a safe and effective vaccine is an effective measure to prevent and control this disease.In this study,virus-like parti-cals of porcine encephalomyocarditis virus were assembled by using the baculovirus-insect cell ex-pression system with P1 and 3CD co-expression strategy,and the immune response effect of the virus in mice was preliminatively evaluated.The results showed that the specific antibody titer of mice in the immune group reached more than 1:192,the neutralizing antibody titer reached more than 1:64,and the combination with ISA206 adjuvant can induce higher antibody levels.The lev-els of IL-4,IFN-γ,IL-5 and TNF-α cytokines all reached 20 pg·mL-1,and were significantly higher than those in PBS group.Therefore,the EMCV virus-like particle vaccine obtained in this study can induce the body to produce good cellular and humoral immune responses,and can also induce the production of inflammatory cytokines,which helps to stimulate the body's immune re-sponse,enhance the protective effect of the vaccine,and provide theoretical basis and technical reserve for the prevention and control of EMCV.
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