畜牧兽医学报2024,Vol.55Issue(10) :4590-4596.DOI:10.11843/j.issn.0366-6964.2024.10.030

猪瘟病毒中和抗体与E2蛋白抗体相关性分析

Correlation Analysis of Neutralizing Antibody and E2 Antibody against Classical Swine Fever Virus

夏应菊 李琰 刘俊 徐嫄 李芳韬 邹兴启 李琪 李佳昕 赵俊杰 张乾义 刘业兵 徐璐
畜牧兽医学报2024,Vol.55Issue(10) :4590-4596.DOI:10.11843/j.issn.0366-6964.2024.10.030

猪瘟病毒中和抗体与E2蛋白抗体相关性分析

Correlation Analysis of Neutralizing Antibody and E2 Antibody against Classical Swine Fever Virus

夏应菊 1李琰 1刘俊 2徐嫄 1李芳韬 1邹兴启 1李琪 1李佳昕 1赵俊杰 1张乾义 1刘业兵 1徐璐1
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作者信息

  • 1. 中国兽医药品监察所,国家猪瘟参考实验室,北京 100081
  • 2. 辽宁省动物疫病预防控制中心,沈阳 110015
  • 折叠

摘要

旨在探索猪瘟病毒中和抗体与E2蛋白抗体的关系,本研究对289份猪血清进行中和抗体效价和E2抗体测定,比较分析二者的相关性;同时从中选取15份血清,测定血清对不同猪瘟病毒流行野毒株的中和效价.结果表明,从整体上说E2抗体水平与中和效价呈正相关.当群体中所有个体E2抗体阻断率均大于60%时,才能保证群体70%以上免疫猪的中和抗体达到保护标准.本研究的结果有助于基层兽医工作者更好地评估临床中猪瘟疫苗的免疫保护状况,降低猪瘟疫情发生风险.

Abstract

In order to explore the correlation between neutralizing antibody and E2 antibody against classical swine fever virus(CSFV),289 swine sera were detected using both neutraliza-tion assay and ELISA among which neutralizing antibody titers of 15 sera were further tested by neutralization assay using different CSFV strains.The results showed that E2 antibody level a-gainst CSFV was positive correlated with neutralizing antibody titer.When the individual per-centage inhibition of E2 antibody was over 60%,the neutralizing antibody titer of more than 70%vaccinated pigs in the herd meet the protection standard.These results could help the local veteri-naries to evaluate the efficacy of CSFV vaccine better in the field and reduce the risk of CSF out-break.

关键词

猪瘟病毒/中和抗体/E2抗体/相关性

Key words

classical swine fever virus/neutralizing antibody/E2 antibody/correlation

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基金项目

国家重点研发计划(2022YFD1800601)

国家重点研发计划(2022YFD1800605)

出版年

2024
畜牧兽医学报
中国畜牧兽医学会

畜牧兽医学报

CSTPCD北大核心
影响因子:0.729
ISSN:0366-6964
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