This experiment was designed to obtain Toxoplasma cysts,which are often used as in-fectious materials in experimental studies related to chronic infection of Toxoplasma gondii,and to search for the most suitable mouse model for cysts passage.In this study,mice with KM,ICR,BALB/c and C57BL/6 strains were intraperitoneally inoculated with different numbers of tachyzoites of PRU strains.After 40 days of infection,antibodies in the serum of surviving mice were detected,and cysts were examined and isolated from the tissues of seropositive mice.Dif-ferent strains of mice were infected by cyst intragastric administration to explore the cysts pas-sage dose and the best passage mouse model.We successfully isolated cysts from the brains of C57BL/6 mice infected with low doses of Toxoplasma tachyzoites.When passing cysts,ICR mice had low mortality and significantly more cysts in the brain than other strains(P＜0.05).C57BL/6 mice can survive and form cysts in the brain when 10-50 tachyzoites are intraperitoneal-ly inoculated;A larger number of toxoplasma cysts could be obtained by intragastric infecting ICR mice with 20-50 cysts.
关键词
PRU株/速殖子/包囊/慢性感染/包囊传代/小鼠模型
Key words
PRU strain/tachyzoite/cysts/chronic infection/cyst passage/mouse model
维普
万方数据