The Mechanism of Chelerythrine against Methicillin-resistant Staphylococcus aureus
The objective of this study was to investigate the antibacterial effect of chelerythrine(CHE)against methicillin-resistant Staphylococcus aureus(MRSA)and approach its mecha-nism.The antibacterial activity of CHE against MRSA was determined by minimum inhibitory concentration(MIC)and minimum bactericidal concentration(MBC).After treatment with CHE,the cell membrane and permeability of MRSA were assessed,and the morphology and ul-trastructure of MRSA cells were observed by electron microscopy to preliminarily analyze the an-tibacterial mechanism of CHE.Additionally,the effect of CHE on reactive oxygen species(ROS)levels and ROS-related factors in MRSA strains was detected,combined with fluorescence quantification and transcriptomics to further explore the antibacterial mechanism of CHE.The results showed that the MIC of CHE against MRSA was 6.25 μg·mL-1,MBC is 12.5 µg·mL-1,and CHE can destroy cell membrane integrity and permeability to achieve antibacterial effect.CHE may block the electronic transmission of respiratory chain and promote ROS production by inhibiting the expression of gene SdhABC and other genes to exert activity against MRSA.In conclusion,this study explored the antibacterial mechanism of CHE against MRSA,which pro-vided a new reference for clinical prevention of MRSA infection.
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