Effects of Methanomassiliicoccus DZ1 on Serum Trimethylamine-N-oxide and Inflammatory Factors,Liver Antioxidant Capacity and Cecum Microbiota in Mice
Methanomassiliicoccales is an indigenous group of methanogens in the mammalian gut that can use trimethylamine(TMA)to produce methane,but the mechanisms of its action on the host remain unclear.The aim of this study was to investigate the effects of Methanomethylophi-lus sp.DZ1 on serum trimethylamine-N-oxide(TMAO)and inflammatory factors,liver antioxi-dant capacity,and cecum microbiota in mice.Fourteen five-week-old male B7/6J mice(body weight 18.4±1.1g)were randomly divided into a control group(n=7)and a treatment group(n=7),single cage feeding.Each mouse in the treatment group was given 200 μL DZ1(1.09× 109 cells·mL-1)daily,and each mouse in the control group was given 200 μL sterile PBS solution daily.The experiment lasted for 4 weeks.The results showed that the body weight and feed in-take of mice in the treatment group had no significant changes(P＞0.05).Serum TMAO con-centration and inflammatory factor levels of mice in the treatment group were significantly de-creased(P＜0.05).The activities of superoxidase dismutase(SOD)in the liver of the treatment group were significantly increased(P=0.035),and the total antioxidant capacity(T-AOC)of the liver was significantly increased(P=0.039).The results of 16S rRNA gene sequencing of cecal bacteria showed no significant difference in bacterial community structure between the treat-ment group and control group(ANOSIM,P=0.161).The relative abundance of Verrucomicro-bia in the treatment group significantly decreased(P=0.064).The relative abundances of Law-sonibacter,Ruminococcus,and Akkermansia were significantly decreased(P＜0.05).In summa-ry,the strain DZ1 had no significant effect on the bacterial community structure of the cecum in mice,but decreased the levels of serum TMAO and inflammatory factors,and increased the total antioxidant capacity of the liver.
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