Effect of Rumen Acidosis on Gastrointestinal Function,Morphology,and Microflora in Goats
This study utilized a model of rumen acidosis induced by the administration of corn flour to investigate its impact on the gastrointestinal tract flora structure of goats.In this experi-ment,eight healthy Boer goats were selected as test subjects,4 in the control group and 4 in the acidosis group after modeling.Gastrointestinal(GI)tissues were collected for HE staining,RT-qPCR,and Western blot assay to investigate the effects of rumen acidosis on the morphology and functional proteins of goat GI tissues.Illumina MiSeq high-throughput sequencing was used to determine the 16S rRNA of the bacteria in rumen fluid and cecum content,aimed to sutdy the effect of acidosis on the bacterial communities in the gastrointestinal tracts.The results showed that,compared with the control group,the tissue morphology and structural integrity of the ru-men and small intestine were damaged in the acidosis group.The relative expression levels of MCTl mRNA and protein in rumen and duodenum were extremely significantly increased(P＜0.01),while the relative expression levels of SLC5A8 mRNA and protein were extremely signifi-cantly decreased(P＜0.01).The results of high-throughput sequencing in rumen contents showed that,the Shannon index of Alpha diversity analysis in the acidosis group was significantly lower than that in the control group(P＜0.01),and the relative richness of Bacteroidetes and Syn-ergistetes was significantly decreased(P＜0.01).The relative abundance of Fibrobacteres and Teneri-cutes was significantly increased(P＜0.05),while the relative abundance of Ruminococcus and Prevo-tella was significantly decreased(P＜0.05).After high-throughput sequencing of cecum contents,com-pared with the control group,the relative richness of Ruminococcus and Oscillospira in acidosis group was significantly decreased(P＜0.05),while the relative richness of Clostridium was significantly in-creased(P＜0.05).In conclusion,rumen acidosis can destroy the integrity of digestive tract morpholo-gy,affect the expression of related proteins,and disturb digestive tract flora structure.
万方数据
维普
