This study aimed to express the nucleocapsid protein(N)of feline infectious peritonitis virus(FIPV)strain SH2021 in vitro and prepare rabbit polyclonal antibodies.Primers were de-signed based on the N gene sequence of FIPV strain SH2021 to construct the recombinant expres-sion plasmid pCold-I-N.Subsequently,the recombinant plasmid was transformed into expres-sion-competent cells BL21(DE3),and expression was induced under conditions of 1.0 mmol·L-1 IPTG and 16 ℃.Finally,purification was performed using a His column,and the purified re-combinant protein was used to immunize New Zealand white rabbits to prepare polyclonal anti-bodies.The experimental results showed that the purified recombinant N protein had a size of ap-proximately 45 ku,and the titer of the prepared rabbit polyclonal antibodies against the N protein reached 1:512 000.The antibodies exhibited good reactivity and specificity towards the N pro-tein.This study successfully prepared rabbit polyclonal antibodies against the FIPV N protein,which showed good reactivity and specificity,providing important tools for FIPV antigen-anti-body detection and research on the biological functions of the N protein.
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