Study on the Susceptibility of CD 163 Gene Knockout Pigs to Actinobacillus pleuropneumoniae,Streptococcus suis,and Haemophilus parasuis
This study aimed to investigate whether pigs with a CD 163 gene knockout(CD 163-KO)resulting from a single base insertion in the SRCR5 domain of the CD 163 gene exhibit al-tered susceptibility to 3 crucial bacterial pathogens compared to their wild-type(WT)counter-parts and to evaluate the biosafety profile of CD 163-KO pigs.This study selected a total of 34 Large White pigs,17 CD 163-KO pigs and 17 wild-type pigs,all at the age of 50 days with similar body weights and health statuses.Among them,2 CD 163-KO Large White pigs and 2 WT Large White pigs were used as blank controls without any infection treatment.None of the pigs had a history of infection or vaccination against Actinobacillus pleuropneumoniae(APP),Streptococ-cus suis(SS),and Haemophilus parasuis(HPS).The pigs were randomly divided into 3 groups of 5 CD 163-KO and 5 WT pigs each:group A received APP serotype 1,group B received SS se-rotype 2,and group C received HPS serotype 13.The pigs were observed continuously for 8 days,the clinical symptoms and mortality were recorded.Upon death,the pigs were promptly necropsied to observe lesions in critical organs such as the heart,thorax,and lungs.Lung sam-ples were collected,and bacterial DNA was extracted from the lungs for quantitative PCR analy-sis.The relative DNA copy numbers of the 3 bacterial species in the lungs of deceased CD163-KO and WT pigs were statistically analyzed and compared.The primary aim of this study was to evaluate whether CD163-KO pigs exhibit altered susceptibility to these 3 key bacterial pathogens compared to their WT counterparts,ultimately assessing the biosafety profile of CD 163-KO pigs.Based on the clinical manifestations observed in the experimental pigs,all groups success-fully contracted the respective bacterial infections,and there were no significant differences in survival rates between CD163-KO and WT pigs within each group(P＞0.05).Furthermore,ac-cording to the pathological findings in the lungs of deceased pigs and the results of quantitative PCR analysis for bacterial DNA,no significant differences were detected in the relative DNA copy numbers of APP serotype 1,SS serotype 2,and HPS serotype 13 strains in the lungs between de-ceased CD163-KO and WT pigs(P＞0.05).The findings of this study serve as robust evidence for the biological safety of CD 163-KO Large White pigs,demonstrating that the knockout of the CD163 gene does not enhance the susceptibility of pigs to APP,SS,and HPS,nor does it signifi-cantly compromise their ability to resist these bacterial infections,which provides strong support for the biological safety assessment of CD163-KO pigs.
CD 163gene knockoutLarge White pigporcine reproductive and respiratory syn-drome virusActinobacillus pleuropneumoniaeStreptococcus suisHaemophilus parasuisbiosafety assessment
万方数据
维普
