Effects of Fibers on Growth Performance,Physiology and Biochemistry,Gastrointestinal Structure and Cecal Microflora of Broilers
This trial was conducted to investigate the effect of different kind of fiber and different adding levels on growth performance,fecal nitrogen metabolism,fecal fiber content,serum bio-chemical index,the gastrointestinal structure and cecal microflora of broilers,in order to provide theoretical basis for the rational utilization of fiber in broilers'diet.Three hundred and sixty one-day-old healthy Shengze"901"male broilers with similar body weight(47.07±0.26 g)were ran-domly allotted into 6 groups with 6 replicates per group and 10 broilers per replicate.The broil-ers were randomly subjected to one of the following 6 treatments for 42 days with 2 periods:con-trol group(T6 group)fed the basal diet,the addition groups were fed a basal diet supplemented with 1.5 kg·t-1 fiber sample 1(T1 group),2.5 kg·t-1 fiber sample 1(T2 group),150 g·t-1 fiber sample 2(T3 group),2 kg·t-1 fiber sample 3(T4 group)and 3 kg·t-1 fiber sample 3(group T5),respectively.The results showed as follows:compared with T6 group,1)The average daily feed intake(ADFI)for 22-42 d and the feed/gain(F/G)for 1-42 d in T2 group were significantly increased by 5.04％and 3.07％(P＜0.05)respectively,the F/G for 22-42 d and 1-42 d in T3 group were significantly increased by 4.16％and 3.68％(P＜0.05)respective-ly,and average body weight(ABW)for 21 d and 42 d,average daily gain(ADG)for 1-21 d and 1-42 d and ADFI for 22-42 d in T5 group were increased significantly by 6.26％and 4.93％,6.70％and 5.02％and 4.87％(P＜0.05)respectively;2)The nitrogen intake and retention(NI and NR)at 21 d in group T1,the nitrogen retention rate(NRR)at 21 d in group T4,the NRR at 21 d in group T5 and the NR at 42 d were significantly increased by 11.24％and 16.77％,6.18％,7.65％and 9.95％,respectively(P＜0.05).Fecal nitrogen content in T5 group at 21 d was significantly decrease d by 6.87％(P＜0.05);3)Urea nitrogen(UN)and total antioxidant capacity(T-AOC)at 42 d in T1 group were significantly increased by 333.33％and 57.14％(P＜0.05)respectively,UR at 21 and 42 d in T2 group were significantly increased by 375.00％and 466.67％respectively,and malondialdehyde(MDA)at 21 d was significantly decreased by 17.69％(P＜0.05).In T3 group,UR at 21 and 42 d was significantly increased by 350.00％and 233.33％respectively,MDA at 21 d was significantly decreased by 15.58％,total protein(TP)and low density lipoprotein(LDL)at 42 d were significantly decreased by 15.34％and 20.00％respectively,and alanine aminotransferase(ALT)was significantly increased by 102.70％(P＜0.05).At 42 d,glucose content in T4 group was significantly increased by 35.36％(P＜0.05),and albumin(ALB),triglyceride(TG)and MDA at 21 d in T5 group were significantly decreased by 10.79％,25.45％and 33.60％,respectively.At 42 d,high density lipoprotein(HDL)and ALT were significantly increased by 25.32％and 62.16％,respectively(P＜0.05);4)At 42 d,the ileum length(IL)in T1 group,the jejunum weight(JW)and IL in T2 group,the jejunum length(JL)and IL in T3 group and IL in T5 group were significantly in-creased by 5.04％,13.71％and 19.26％,19.38％and 21.84％and 23.18％,respectively(P＜0.05);5)The villus height(VH)of ileum in T3 group was significantly decreased by 29.20％(P＜0.05)at 42 d,the crypt depth(CD)of duodenum and jejunum in T5 group were significant-ly increased by 116.67％and 73.33％,respectively,and the VH/CD of duodenum was signifi-cantly decreased by 48.02％(P＜0.05).6)Alpha and Beta diversity analysis showed that cae-cum microflora has obvious difference between each group.The type and quantity of microbes OTU for 21 d was increased.Bacteroides fragilis A and Alistipes finegoldii in genus of Bacte-roides and Allstilpes were the most abundant and were the dominant genera.Bacteroides and Bacteroides fragilis A were the highest richness at 21 days,and Allstilpes and Alistipes finegol-dii were the highest richness at 42 days.It mainly played a functional role in carbohydrate metab-olism and amino acid metabolism,and transport and replication and repair by KEGG pathway a-nalysis.In conclusion,dietary fiber supplementation can promote intestinal development,im-prove and stabilize intestinal microflora,improve nutrient utilization,and thus increase daily gain and feed intake of broilers.Under the conditions of this experiment,adding 3 kg·t-1 Muzhiqian product has the best effect.
万方数据
维普
