Isolation and Identification of Three Lumpy Skin Disease Viruses in China and Their GPCR Gene Analysis
This study was designed to understand the prevalence and genetic evolution of field strains of lumpy skin disease(LSD)in cattle in our country,and determine the biological charac-teristics of lumpy skin disease virus(LSDV).This study conducted PCR testing,virus isolation,immunofluorescence identification,electron microscopy observation,GPCR gene sequencing,and genetic evolution analysis on skin nodule samples from cattle suspected of having lumpy skin disease.The results indicate that the disease affecting cattle in three provinces in our country is Lumpy Skin Disease.Three LSDV strains were isolated using primary lamb testicular cells(PLT)and named LSDV/Heilongjiang/2022,LSDV/Jiling/2022,and LSDV/Jiangxi/2022.The growth curve of the three viruses showed the fastest growth rate in the first 96 hours,with the highest titer at 96 to 120 hours.Afterward,the virus growth rate started to slow down,and the highest titer was reached 96 hours after inoculation,the titers for the three strains were 106.0 TCID50·mL-1,105.3 TCID50·mL-1,and 105.1 TCID50·mL-1,respectively.Under transmis-sion electron microscopy,virus particles appeared elliptical,with a diameter between 200 and 300 nm.Genetic evolution analysis of the GPCR gene sequences of the three strains,along with prev-alent strains in various regions of China and other countries in recent years,showed that the three isolated strains and the prevalent strains in various regions of China are on the same branch.This indicates a strong correlation in the prevalence of Lumpy Skin Disease in various regions of Chi-na.This study successfully isolated three strains of LSDV from skin nodules of cattle in Hei-longjiang,Jilin,and Jiangxi provinces,enriching the epidemiological and pathogenic data of LS-DV in China.It holds significant importance for the continuous monitoring of LSDV and the de-velopment of new vaccines.
lumpy skin disease virusisolation and identificationbiological characteristicsphy-logenetic analysis
万方数据
维普
