Effect of Bluetongue Virus Infection on Type Ⅰ Interferon Responses in Sheep Lung Microvascular Endothelial Cells
Bluetongue virus(BTV)is an arbovirus that seriously harms ruminants such as Sheep,while sheep lung microvascular endothelial cells(SLMECs)constitute a semi-selective barrier in order to study the molecular mechanism of interferon antiviral immune response in BTV infection and SLMECs.In this study,BTV plaque assay was used to induce SLMECs at 18 h,24 h and 36 h with multiple infection(MOI)1.The mRNA expression characteristics of interferon-related pathway genes were analyzed by real-time fluorescence quantitative PCR,and the protein expres-sions of MDA5,TRAF3,RIG-I and TBK1 were analyzed by Western blot.The results showed that the changes of interferon signaling pathway gene expression were most obvious,and the mR-NA expressions of RIG-I,MDA5,IKKε,IFN-β,TBK1 and TRAF6 genes were up-regula-ted,and the differences were extremely significant(P＜0.01)at 36 h of BTV induction,while the mRNA expressions of IFN-α,VISA and USP18 genes were significantly downregulated(P＜0.01).At the protein level,the protein expressions of MDA5,TBK1,RIG-I and TRAF3 were mainly upregulated.This study found that BTV infection can induce an IFN immune re-sponse of SLMECs,which lays a foundation for further analysis of the mechanism of IFN-Ⅰ sig-naling pathway activation in the innate immune response against BTV infection.
万方数据
维普
