Isolation of a Novel Duck Reovirus Strain and Establishment of Indirect ELISA for Detecting Antibodies
Novel duck reovirus(NDRV),which can infect ducklings of many breeds,leads to hemorrhagic necrosis of liver and spleen and cause immunosuppression,and has caused serious economic losses to Chinese waterfowl farming industry.However,there are still no commercial test kits on the market.In this study,a pure new duck reovirus was successfully isolated from clinical materials by plaque purification,named as E232 strain.And by using σC protein ex-pressed in prokaryotes as the coating antigen,an indirect ELISA was established to detect NDRV se-rum antibodies.The genetic evolution results show that E232 belongs to NDRV and has 96.4％-98.9％nucleotide homology with other NDRV strains.Animal regression test showed that the incidence of E232 is 100％.The spleens of the affected ducklings showed severe swelling,bleeding and necrosis,and the cloacal detoxification and pharynx detoxification reached the peak on the 5th day after infection.This assay detected NDRV antibody titer in positive sera at 1∶6 400 dilution,the coefficients of variation of intra batch and inter batch repeatability tests were 1.53％-7.24％and 1.84％-8.30％.It showed 80.93％positive rate for 236 clinical serum samples but did not react with any other disease positive sera.In conclusion,the successful isolation of the E232 strain in this study has enriched the epidemiological information database of NDRV in China.The success-ful establishment of the indirect ELISA provides a good technical means for the prevention and control of NDRV.
novel duck reovirusisolation and identificationσC proteinindirect ELISA
万方数据
维普
