Effects of Biomineralization on the Biological Characteristics and Immunogenicity of the LaSota Strain of Newcastle Disease Virus
This study aims to investigate the effects of biomineralization on the biological charac-teristics and immunogenicity of the Newcastle disease virus(NDV)LaSota strain,and provide new insights for the development of heat-stable live vaccines.The optimal biomineralization con-ditions for NDV were determined by measuring viral particle size,hemagglutination titers,and spot blots under different mineralization conditions.The effect of mineralization on viral replica-tion was evaluated by inoculating mineralized viruses into BHK-21 cells,and then comparing their titer changes after heat treatment.The immunogenicity of mineralized viruses was evaluated through virus neutralization tests.Finally,the immunological efficacy of mineralized viruses was assessed in SPF chicks via water administration.Results were as follows:Upon optimization of the biomineralization conditions,the LaSota strain exhibited the best mineralization effect under 4 mmol·L-1 Na2HPO4 and 3 mmol·L-1 CaCl2 conditions,characterized by the largest particle size of the mineralized virus,the lowest hemagglutination titer,and a high mineralization rate of 98.25％.Compared to the non-mineralized virus,the mineralized LaSota strain showed a delayed onset of replication in BHK-21 cells,however,there was no significant difference in the final rep-lication titers between them.Following neutralization with NDV antibodies,the titer reduction value of the mineralized virus was 103.0 TCID50·mL-1,which was significantly lower than that of the non-mineralized group.After incubation at 56 ℃ for 15 minutes,the viral titer of the min-eralized LaSota strain decreased by only 103.5 TCID50·mL-1,demonstrating a heat resistance characteristic comparable to that of the TS09-C heat-resistant strain.Fourteen days post-immuni-zation,antibody levels in SPF chicks immunized with the mineralized LaSota strain were higher than those in chicks receiving the non-mineralized LaSota strain.Challenge experiments conduc-ted 42 and 78 days post-immunization showed survival rates of 100％and 60％,respectively,in the group immunized with the mineralized LaSota strain.This study optimized the best biominer-alization conditions for the NDV LaSota strain.Mineralization delays the initial viral replication but does not affect the final titer.After mineralization,it can reduce neutralization reaction of vi-rus with antibodies and enhance thermal stability of virus,providing good protective effects to SPF chicks via drinking water immunization.This study confirms that developing heat-stable live vaccines for Newcastle disease through biomineralization is a practical and feasible approach,and also offers valuable references and inspirations for developing heat-stable live vaccines for other viruses.
LaSota strain of Newcastle disease virusbiomineralizationbiological characteris-ticsthermal stabilityimmunogenicity
万方数据
维普
