Analysis of Drug Resistance and Pathogenicity of Metallo-β-Lactamase-producing Porcine ST201 Pasteurella multocida
The study was design to investigate the pathogenic agents of respiratory tract diseases of fattening pigs in a pig breeding enterprise in Jingzhou,Hubei Province.The pathogen was screened and isolated from the diseased pig lung tissue.Morphological observation,physiological and biochemical analysis,16S rDNA gene sequence,serotype typing,multilocus sequence typ-ing,drug sensitivity test,drug resistance gene sequencing analysis and animal pathogenicity test were performed for the isolated strains.The results showed that the isolated strain Pm0525 was Gram-negative brevibacterium.It was identified as Pasteurella multocida by biochemical assay,PCR and 16S rDNA sequence analysis.The isolated strain was identified as Pasteurella multoci-da type A.Multilocus sequence typing analysis confirmed that the isolated strain is type ST201 and merged with type ST1615.The isolated strain was sensitive 13 kinds of antibiotics,such as gentamicin,kanamycin and medemycin,and resistant to chloramphenicol,clindamycin,cefu-roxime,ceftazidime and tetracycline.The isolated strain showed moderate biofilm formation ability,carrying out four resistance genes,Sul Ⅰ,Sul Ⅱ,tetA and bla-TEM.The isolated strain may play the role of anti-β-lactam antibiotics by producing metal-beta-lactamase(MBL).In addition,traditional Chinese medicine Coptis had obvious inhibitory effect on the isolated strain in vitro.The isolated strain carried 18 virulence genes,and showed strong pathogenicity to mice.This study systematically analyzed the serotype,molecular typing,drug sensitivity and pathogenicity of swine Pasteurella multocida,providing scientific basis and data reference for further research on the transmission,prevention and precise treatment of Pasteurella infection.
万方数据
维普
