Characteristics and Phylogenetic Analysis of Mitochondrial Genome in the Ligula intestinalis
This experiment was conducted to obtain the complete mitochondrial genome sequence of Ligula intestinalis,revealed the sequence's structural features,and explored the phylogenetic information of L.intestinalis.Several L.intestinalis were obtained from Pseudorasbora parva and genomic DNA was extracted.The complete mitochondrial genome of L.intestinalis was se-quenced,assembled,and annotated using Illumina sequencing technology after quality control of the DNA,followed by bioinformatic analysis.Maximum likelihood(ML)and bayesian inference(BI)analysis were used to construct the phylogenetic tree.The results showed that the complete mitochondrial genome of L.intestinalis was 13 655 bp in length,and its A+T content was 67.6％,exhibiting a clear AT bias.The complete mitochondrial genome was composed of 12 protein-coding genes(PCGs),22 tRNA genes,two rRNA genes and two NCRs(non-coding re-gions).Among the 12 PCGs,11 used ATG as the start codon,except for cox 3,which had a TTG initial one.Among the termination codons,five out of twelve were identified as TAA,five as TAG,while neither the cox3 nor nad3 genes had termination codons.The total length of the 22 tRNA genes was 1 272 bp.Most tRNAs have a conventional cloverleaf structure,but trnS1 and trnR lack dihydrouridine arms of tRNA.The nad2,nad6 and nad4 genes are more suitable as molecular markers for L.intestinalis.The results of the phylogenetic tree showed that Ligula and Digramma were most closely related to each other,forming a sister group with Dibothrio-cephalus.The complete mitochondrial genome of L.intestinalis was obtained.This study will provide a reference for the study of the taxonomy and systematics of L.intestinalis.
万方数据
维普
