Effect of Recombinant Serpins of Psoroptes ovis on Psoriasis-like Skin Inflammation in Mice
Our team previously found that recombinant serine protease inhibitors 4 and 5(rPssoSP4 and rPssoSP5)of Psoroptes ovis could inhibit the enzymatic activities of chymotrypsin,trypsin and elastase in vitro,thus we speculated that these two recombinant proteins might have anti-inflammation activities.To confirm the above speculation,we assessed whether rPsoSP4 and rPsoSP5 could improve psoriatic-like skin inflammation in mice.BALB/c mice were random-ly divided into 5 groups(n=6),Vaseline or imiquimod cream(IMQ)was applied daily on the back skin continuously for 6 days to establish the control group and psoriasis model groups.Dur-ing this period,the psoriasis model groups were intradermal injected with PBS(IMQ group)or pET32a(+)-vector protein(IMQ+pET32a(+)group)or rPsoSP4(IMQ+rPsoSP4 group)or rPssoSP5(IMQ+rPssoSP5 group),then the changes of psoriasis-like skin lesions were observed every day,and mice were sacrificed on the 7th day.Subsequently,the pathological changes and the transcription levels of IL-1β,IL-6,and TNF-α in the skin lesions among each group were compared.Results showed that IMQ induced psoriasis-like lesions were successfully established,and the mice appeared typical psoriasis-like lesions.Compared with the model group(IMQ group),IMQ+rPsoSP4 group can effectively attenuated psoriasis-like skin lesions,including significant inhibition of weight loss(from 4th to 6th day,P＜0.05,P＜0.01,P＜0.001)and re-ductions of skin erythema(5th and 6th day),skin scales(from 4th to 6th day),skin thickness(from 4th to 6th day)and PASI score(from 3rd to 6th day)in mice(P＜0.05,P＜0.001).Additionally,compared with the model group(IMQ group),IMQ+rPsoSP4 group showed that the pathologi-cal damage in the skin lesion was significantly reduced,accompanied by the significant decrease in epidermal thickness(P＜0.05)and the number of infiltrated inflammatory cells(P＜0.001).Moreover,there was a significantly decreased in mRNA levels of IL-1β,IL-6,and TNF-α in the skin lesion of IMQ+rPsoSP4 group in comparison with IMQ groups,while IMQ+rPsoSP5 group had no significant improvement on psoriatic lesions.In conclusion,rPsoSP4 could signifi-cantly improve psoriasis skin lesion in mice and has significant anti-inflammatory activity,while rPssoSP5 seemed to be no obvious effect.
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维普
