Preparation of Monoclonal Antibodies against Feline Calicivirus VP1 Protein and Identification of Antigenic Epitopes
The purpose of this study was to prepare and identify monoclonal antibodies(mAbs)a-gainst the VP1 protein of feline calicivirus(FCV)strain SH14.In this study,the whole virus of the SH14 strain of FCV was used as an immunogen to immunize BALB/c mice through intraper-itoneal injection,and mAbs against the VP1 protein were prepared.Through ELISA screening,a specific monoclonal antibody clone,3A3C1,was successfully obtained.A comprehensive biologi-cal characterization of the 3A3C1 clone mAb was conducted,confirming it as an IgG1 type with aκ light chain.The results of Western blot and immunofluorescence assay(IFA)showed that the 3A3C1 clone mAb could specifically recognize and bind to FCV strains SH14,F9,and GZ22.In addition,this study further explored the linear antigenic epitope of the 3A3C1 clone mAb.By segmentally expressing the VP1 protein and conducting detailed Western blot analysis,the linear antigenic epitope of the 3A3C1 clone mAb was finally determined to be located in the426 PSRLTPAGDYAITSG440 region of the VP1 protein.The 3A3C1 clone mAb prepared in this study is not only an important tool for understanding the immunological characteristics of FCV but also has potential applications in developing FCV diagnostic methods and vaccine strategies.
feline calicivirusmonoclonal antibodyantigenic epitopecapsid protein
万方数据
维普
