Effects of Modified Zhizhu Powder on Intestinal Mucosal Barrier and Antioxidant Capacity of Weaned Rabbits
The study aimed to investigate the effects of Modified Zhizhu Powder on the ileal intes-tinal mucosal barrier of weaned rabbits,with the goal of providing new ideas for the prevention and treatment of diarrhea diseases in weaned rabbits.Ninety 35-day-old New Zealand white rab-bits,with similar body weights,were randomly divided into 5 groups,based on the principle of 50/50 male and female.Rabbits in control group were fed a basal diet,Enrofloxacin group,low-dose group,medium-dose group and high-dose group were fed the basal diet supplemented with 0.2％Enrofloxacin powder and 0.1％,0.3％and 0.5％Modified Zhizhu Powder,respectively.The pre-test lasted for 7 days,and the formal test lasted for 14 days.The integrity of the intesti-nal barrier and its antioxidant capacity were measured after the experiment.The results showed that adding Modified Zhizhu Powder could significantly reduce the intestinal pathological tissue score(P＜0.05).Compared with the blank group,the other treatment groups all significantly increased the relative expression level of ZO2(zonula occludens protein 2)mRNA in the ileum(P＜0.001).The high-dose group significantly increased the mRNA levels of Occludin,clau-din,ZO1(zonula occludens protein 1),ZO2,and JAM2(junctional adhesion molecule 2)(P＜0.001).Modified Zhizhu Powder significantly increased the expression of MUC2(Mucin 2)pro-tein in the ileum(P＜0.05)and the high-dose group significantly reduced MPO(myeloperoxi-dase)activity(P＜0.01).Modified Zhizhu Powder high-doce group could significantly enhance the secretion of sIgA,reduce the levels of pro-inflammatory cytokines IL-1β,IL-2,IL-6,and TNF-α,and increase the levels of anti-inflammatory cytokines IL-10 and TGF-β.Modified Zhizhu Powder could also improve the activity of antioxidant enzymes(SOD,GSH,T-AOC,CAT).In conclusion,the Modified Zhizhu Powder could effectively improve the morphology and structure of the ileum of weaned rabbits,strengthen the tight connection between intestinal cells,maintain the permeability of intestinal mucosa,reduce the inflammatory response of the intestinal tract,enhance the function of the intestinal mucosal barrier,and play an antioxidant role,which can have a positive effect on the use of substitute antibodies.
weaning diarrhea of young rabbitsModified Zhizhu Powderintestinal mucosal bar-rierinflammatory responseantioxidation
万方数据
维普
