Effect of Modified Yuyin Decoction on cGAS-STING Pathway of African Swine Fever Virus Infected PAMs
In order to evaluate the efficacy of Chinese traditional medicine against African swine fever virus(ASFV)in vitro,five experimental drugs were used in this study.The effects of dif-ferent Chinese herbal medicines on ASFV encored capshell protein p72 in porcine alveolar macro-phages(PAMs)infected with African swine fever virus were detected by qPCR.Modified Yuyin decoction(MYY)was selected as a Chinese medicine that could significantly reduce the expres-sion of ASFV-p 72 gene.The main components of anti-ASFV were detected by LC-MS analysis.CCK8 method was used to observe the effect of modified Yuyin decoction on the activity of PAMs.The expression of ASFV-p72 gene was detected by fluorescence quantitative PCR.West-ern blot and ELISA were used to detect CGAS-STING signaling pathway protein expression.RU.521 was used as a cGAS inhibitor to verify the anti-ASFV effect of MYY in the CGAS-STING pathway.The results showed that MYY can significantly reduce the expression of ASFV-p72 gene in PAMs,and the best effect is given at 2 h after ASFV infection,and it has a concen-tration-dependent improvement effect on the cell viability after ASFV infection of PAMs.The expression levels of interferon gene stimulating protein(STING),TANK binding kinase 1(TBK1),interferon regulatory factor 3(IRF3)and interferon induced transmembrane protein 3(IFITM3)in the supernatant of PAMs cells were decreased by MYY 2 h after the challenge.The expression of cyclic GMP-AMP synthetase(cGAS)and interferon β(IFNβ)protein in PAMs cells increased,and MYY could activate the cGAS-STING-TBK1-IRF3-IFNβsignaling pathway and promote the expression of IFITM3.After treatment with RU.521,MYY could still increase cell viability and decrease the expression of ASFV-p72 gene.In summary,MYY has a potential anti-ASFV effect,which may be related to the activation of cGAS-STING signaling pathway to promote the expression of IFITM3.
万方数据
维普
